Application
Immunohistochemical analysis of paraffin-embedded BT483 xenograft, using C5orf54 antibody at 1:500 dilution.Application
Sample (30 ug of whole cell lysate) A: H1299 B: MCF-7 7.5% SDS PAGE diluted at 1:1000Application
C5orf54 antibody detects C5orf54 protein at mitochondria by immunofluorescent analysis. Sample: H1299 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: C5orf54 protein stained by C5orf54 antibody diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 μm.| Product Name | C5orf54 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to C5orf54 (chromosome 5 open reading frame 54) |
| Immunogen | Recombinant protein encompassing a sequence within the C-terminus region of human C5orf54. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | Human |
| Predicted Reactivity | BovineBovine (91%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | Buster3 antibody C5orf54 antibody transposon-derived Buster3 transposase-like protein antibody chromosome 5 open reading frame 54 antibody |
|---|---|
| Molecular Weight(MW) | 68 kDa (note) (3) |
Application
Immunohistochemical analysis of paraffin-embedded BT483 xenograft, using C5orf54 antibody at 1:500 dilution.
Application
Sample (30 ug of whole cell lysate) A: H1299 B: MCF-7 7.5% SDS PAGE diluted at 1:1000
Application
C5orf54 antibody detects C5orf54 protein at mitochondria by immunofluorescent analysis. Sample: H1299 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: C5orf54 protein stained by C5orf54 antibody diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 μm.| Positive Control | H1299 , MCF-7 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
