Application
Sample (30 µg of whole cell lysate) A: Non-transfected 293T lysates B: C9orf72 transfected 293T lysate 10% SDS PAGE diluted at 1:5000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
Sample (30 µg of whole cell lysate) A: U87-MG 12% SDS PAGE diluted at 1:2000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
C9orf72 antibody detects C9orf72 protein at cytoplasm and nucleus by immunofluorescent analysis. Sample: U87-MG cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: C9orf72 protein stained by C9orf72 antibody diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 μm.Application
C9orf72 antibody detects C9orf72 protein at on mouse fore brain by immunohistochemical analysis. Sample: Paraffin-embedded mouse fore brain. C9orf72 antibody dilution: 1:500.Application
Sample (30 µg of whole cell lysate) A: Neuro2A 10% SDS PAGE diluted at 1:2000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
C9orf72 antibody detects C9orf72 protein at cytoplasm in human colon cancer by immunohistochemical analysis. Sample: Paraffin-embedded human colon cancer. C9orf72 antibody diluted at 1:500.| Product Name | C9orf72 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to C9orf72 (uncharacterized protein C9orf72) |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human C9orf72. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHCIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | HumanMouse |
| Predicted Reactivity | Xenopus laevisXenopus laevis (90%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | MGC23980 antibody RP11-27J8.2 antibody C9orf72 antibody uncharacterized protein C9orf72 antibody chromosome 9 open reading frame 72 antibody |
|---|---|
| Molecular Weight(MW) | 54 kDa (note) (4) |
Application
Sample (30 µg of whole cell lysate) A: Non-transfected 293T lysates B: C9orf72 transfected 293T lysate 10% SDS PAGE diluted at 1:5000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
Sample (30 µg of whole cell lysate) A: U87-MG 12% SDS PAGE diluted at 1:2000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
C9orf72 antibody detects C9orf72 protein at cytoplasm and nucleus by immunofluorescent analysis. Sample: U87-MG cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: C9orf72 protein stained by C9orf72 antibody diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 μm.
Application
C9orf72 antibody detects C9orf72 protein at on mouse fore brain by immunohistochemical analysis. Sample: Paraffin-embedded mouse fore brain. C9orf72 antibody dilution: 1:500.
Application
Sample (30 µg of whole cell lysate) A: Neuro2A 10% SDS PAGE diluted at 1:2000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
C9orf72 antibody detects C9orf72 protein at cytoplasm in human colon cancer by immunohistochemical analysis. Sample: Paraffin-embedded human colon cancer. C9orf72 antibody diluted at 1:500.| Positive Control | U87-MG , Neuro2A , C9orf72 transfected 293T lysate |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
