Mouse monoclonal antibody [X325] to CA125CA125/MUC16 antigen is a serum marker that is used routinely in gynecologic practice to monitor patients with ovarian cancer. It is a mullerian duct differentiation antigen that is overexpressed in epithelial ovarian cancer cells and secreted into the blood, although its expression is not entirely confined to ovarian cancer. Most biochemical studies have concluded that CA125/MUC16 is a high molecular mass glycoprotein, although estimates of its size range from 200 to 2000 kDa with smaller.
Immunogen
Full length native protein (purified from human ovarian carcinoma).
Key Feature
Clonality
Monoclonal
Isotype
IgG1
Clone Number
X325
Host Species
Mouse
Tested Applications
ELISAIHC-PWB
For ELISA: Use at an assay dependent dilution. For IHC-P: Use at an assay dependent dilution. For WB: Use at an assay dependent dilution. Optimal dilutions/concentrations should be determined by the researcher.:
Species Reactivity
Human
Concentration
1mg/ml
Purification
Affinity purified
Target Information
Gene Synonyms
FLJ14303 606154 94025 MUC16 CA125 Q8WXI7
Target
CA125
Tissue Specificity
Epitope specificity group B (ISOBM classification).
Application Notes
For ELISA: Use at an assay dependent dilution. For IHC-P: Use at an assay dependent dilution. For WB: Use at an assay dependent dilution. Optimal dilutions/concentrations should be determined by the researcher.:
Additional Information
Form
Liquid
Storage Instructions
Keep as concentrated solution, aliquot and store at 4°C. Do not freeze.
Storage Buffer
10 mM Tris, pH 7.5, 0.15 M NaCl, and 0.05% sodium azide