Application
U87-MG whole cell and nuclear extracts (30 µg) were separated by 5% SDS-PAGE, and the membrane was blotted with CA150 antibody diluted at 1:500.Application
CA150 antibody detects CA150 protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: CA150 protein stained by CA150 antibody diluted at 1:500. Red: phalloidin, a cytoskeleton marker, diluted at 1:50.Application
Immunohistochemical analysis of paraffin-embedded human colon, using CA150 antibody at 1:250 dilution.Application
CA150 antibody detects CA150 protein at nucleus in mouse brain by immunohistochemical analysis. Sample: Paraffin-embedded mouse brain. CA150 antibody diluted at 1:500.Application
CA150 antibody detects CA150 protein at nucleus in rat testis by immunohistochemical analysis. Sample: Paraffin-embedded rat testis. CA150 antibody diluted at 1:500.Application
HeLa whole cell and nuclear extracts (30 µg) were separated by 5% SDS-PAGE, and the membrane was blotted with CA150 antibody diluted at 1:500.| Product Name | CA150 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to CA150 (transcription elongation regulator 1)This gene encodes a nuclear protein that regulates transcriptional elongation and pre-mRNA splicing. The encoded protein interacts with the hyperphosphorylated C-terminal domain of RNA polymerase II via multiple FF domains, and with the pre-mRNA splicing factor SF1 via a WW domain. Alternative splicing results in multiple transcripts variants encoding different isoforms. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human CA150. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | HumanMouseRat |
| Predicted Reactivity | Mouse, RatMouse (81%), Rat (81%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | CA150 antibody MGC133200 antibody TAF2S antibody Urn1 antibody TCERG1 antibody TATA box-binding protein-associated factor 2S antibody transcription elongation regulator 1 antibody co-activator of 150 kDa antibody transcription factor CA150 antibody |
|---|---|
| Molecular Weight(MW) | 124 kDa (note) (2) |
| Cellular Localization | Nucleus |
Application
U87-MG whole cell and nuclear extracts (30 µg) were separated by 5% SDS-PAGE, and the membrane was blotted with CA150 antibody diluted at 1:500.
Application
CA150 antibody detects CA150 protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: CA150 protein stained by CA150 antibody diluted at 1:500. Red: phalloidin, a cytoskeleton marker, diluted at 1:50.
Application
Immunohistochemical analysis of paraffin-embedded human colon, using CA150 antibody at 1:250 dilution.
Application
CA150 antibody detects CA150 protein at nucleus in mouse brain by immunohistochemical analysis. Sample: Paraffin-embedded mouse brain. CA150 antibody diluted at 1:500.
Application
CA150 antibody detects CA150 protein at nucleus in rat testis by immunohistochemical analysis. Sample: Paraffin-embedded rat testis. CA150 antibody diluted at 1:500.
Application
HeLa whole cell and nuclear extracts (30 µg) were separated by 5% SDS-PAGE, and the membrane was blotted with CA150 antibody diluted at 1:500.| Positive Control | HeLa , HeLa nuclear extract , U87-MG , U87-MG nuclear extract |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
