Application
Immunohistochemical analysis of paraffin-embedded human hepatoma, using c-Abl antibody at 1:500 dilution.Application
Sample (30 ug of whole cell lysate) A: Jurkat 5% SDS PAGE diluted at 1:500Application
c-Abl antibody detects c-Abl protein at cytoplasm and nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: c-Abl protein stained by c-Abl antibody diluted at 1:100. Blue: Hoechst 33342 staining. Scale bar = 10 μm.| Product Name | c-Abl antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to c-Abl (c-abl oncogene 1, receptor tyrosine kinase)The ABL1 protooncogene encodes a cytoplasmic and nuclear protein tyrosine kinase that has been implicated in processes of cell differentiation, cell division, cell adhesion, and stress response. Activity of c-Abl protein is negatively regulated by its SH3 domain, and deletion of the SH3 domain turns ABL1 into an oncogene. The t(9;22) translocation results in the head-to-tail fusion of the BCR (MIM:151410) and ABL1 genes present in many cases of chronic myelogeneous leukemia. The DNA-binding activity of the ubiquitously expressed ABL1 tyrosine kinase is regulated by CDC2-mediated phosphorylation, suggesting a cell cycle function for ABL1. The ABL1 gene is expressed as either a 6- or 7-kb mRNA transcript, with alternatively spliced first exons spliced to the common exons 2-11. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the C-terminus region of human c-Abl. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
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| Species Reactivity | Human |
| Predicted Reactivity | Mouse, Rat, BovineMouse (83%), Rat (84%), Bovine (86%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | ABL antibody JTK7 antibody bcr/abl antibody c-ABL antibody p150 antibody v-abl antibody ABL1 antibody proto-oncogene tyrosine-protein kinase ABL1 antibody Abelson tyrosine-protein kinase 1 antibody proto-oncogene c-Abl antibody v-abl Abelson muri |
|---|---|
| Molecular Weight(MW) | 123 kDa (note) (2) |
| Cellular Localization | Cytoplasm , cytoskeleton , Nucleus |
Application
Immunohistochemical analysis of paraffin-embedded human hepatoma, using c-Abl antibody at 1:500 dilution.
Application
Sample (30 ug of whole cell lysate) A: Jurkat 5% SDS PAGE diluted at 1:500
Application
c-Abl antibody detects c-Abl protein at cytoplasm and nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: c-Abl protein stained by c-Abl antibody diluted at 1:100. Blue: Hoechst 33342 staining. Scale bar = 10 μm.| Positive Control | Jurkat |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
