Application
Western blot analysis of Calcineurin A in lysate from Jurkat cells (Lane 1) and rat kidney (Lane 2).| Product Name | Calcineurin A antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal antibody to Calcineurin ACalcineurin is a calcium/calmodulin-dependent serine/threonine protein phosphatase. The active enzyme is a heterodimer consisting of a catalytic A subunit of 61 kDa and a myristylated regulatory B subunit of 19 kDa that binds calcium. The catalytic A subunit consists of an amino terminal catalytic domain, followed by calmodulin and B subunit binding sites, and a caboxyl-terminal autoinhibitory domain. Calnineurin is the target of immunosuppressant drugs such as cyclosporin A and FK506. Calcineurin controls the translocation of nuclear factors of activated T cells (NFAT), a family of transcriptional activators that control the expression of cytokine genes essential to immune response, from the cytosol into the nucleus of activated T cells. Calcineurin has been proposed to play an important role in mediating calcium-activated cell death. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IPWB |
| Western Blotting (1-4 µg/ml) and for immunoprecipitation (10-20 µg/ml). However, the optimal conditions should be determined individually. The immunoaffinity purified antibody detects a 61 kDa band, corresponding to the expected molecular weight of Calcineurin A on immunoblots. A 32 kDa band can also be detected in human samples, presumably to be the cleavage product of Calcineurin A..: |
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| Species Reactivity | HumanMouseRatHamsterRabbitBovine |
| Concentration | 0.2mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | PPP3CA CALN CALNA CCN1 PPP3CB PPP2B CNA1 CALNA1 |
|---|---|
| Target | Calcineurin A |
| Tissue Specificity | This antibody is specific for Calcineurin A. |
Application
Western blot analysis of Calcineurin A in lysate from Jurkat cells (Lane 1) and rat kidney (Lane 2).| Application Notes | Western Blotting (1-4 µg/ml) and for immunoprecipitation (10-20 µg/ml). However, the optimal conditions should be determined individually. The immunoaffinity purified antibody detects a 61 kDa band, corresponding to the expected molecular weight of Calcineurin A on immunoblots. A 32 kDa band can also be detected in human samples, presumably to be the cleavage product of Calcineurin A..: |
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| Form | Liquid |
|---|---|
| Storage Instructions | Aliquot and store at -20ºC .The antibody solution should be gently mixed before use. |
| Storage Buffer | PBS (pH 7.2) containing 30% glycerol, 0.5% BSA, 0.01% thimerosal |
