Application
Sample (30 µg of whole cell lysate) A: HeLa B: HepG2 12% SDS PAGE diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
Immunohistochemical analysis of paraffin-embedded SW480 xenograft, using Calcium binding protein P22 antibody at 1:100 dilution.Application
Calcium binding protein P22 antibody detects CHP protein by western blot analysis. A. 50 µg rat liver lysate/extract 12% SDS-PAGE Calcium binding protein P22 antibody dilution: 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
Calcium binding protein P22 antibody detects CHP protein by western blot analysis. A. 50 µg mouse liver lysate/extract 12% SDS-PAGE Calcium binding protein P22 antibody dilution: 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
Calcium binding protein P22 antibody detects Calcium binding protein P22 protein at cytosol on mouse lung by immunohistochemical analysis. Sample: Paraffin-embedded mouse lung. Calcium binding protein P22 antibody dilution: 1:500.Application
Calcium binding protein P22 antibody detects Calcium binding protein P22 protein at cytoplasm by immunofluorescent analysis. Sample: HepG2 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Calcium binding protein P22 protein stained by Calcium binding protein P22 antibody diluted at 1:500. Blue: Hoechst 33342 staining.| Product Name | Calcium binding protein p22 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to Calcium binding protein p22This gene encodes a phosphoprotein that binds to the Na+/H+ exchanger NHE1. This protein serves as an essential cofactor which supports the physiological activity of NHE family members and may play a role in the mitogenic regulation of NHE1. The protein shares similarity with calcineurin B and calmodulin and it is also known to be an endogenous inhibitor of calcineurin activity. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human Calcium binding protein p22. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
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| Species Reactivity | HumanMouseRat |
| Predicted Reactivity | Zebrafish, Xenopus laevis, Chicken, Rhesus Monkey, BovineZebrafish (86%), Xenopus laevis (90%), Chicken (96%), Rhesus Monkey (100%), Bovine (99%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | SLC9A1BP antibody CHP antibody SLC9A1 binding protein antibody calcineurin homologous protein antibody calcineurin B homolog antibody calcium-binding protein CHP antibody calcium-binding protein p22 antibody |
|---|---|
| Molecular Weight(MW) | 22 kDa (note) (2) |
| Cellular Localization | Cytoplasm |
Application
Sample (30 µg of whole cell lysate) A: HeLa B: HepG2 12% SDS PAGE diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
Immunohistochemical analysis of paraffin-embedded SW480 xenograft, using Calcium binding protein P22 antibody at 1:100 dilution.
Application
Calcium binding protein P22 antibody detects CHP protein by western blot analysis. A. 50 µg rat liver lysate/extract 12% SDS-PAGE Calcium binding protein P22 antibody dilution: 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
Calcium binding protein P22 antibody detects CHP protein by western blot analysis. A. 50 µg mouse liver lysate/extract 12% SDS-PAGE Calcium binding protein P22 antibody dilution: 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
Calcium binding protein P22 antibody detects Calcium binding protein P22 protein at cytosol on mouse lung by immunohistochemical analysis. Sample: Paraffin-embedded mouse lung. Calcium binding protein P22 antibody dilution: 1:500.
Application
Calcium binding protein P22 antibody detects Calcium binding protein P22 protein at cytoplasm by immunofluorescent analysis. Sample: HepG2 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Calcium binding protein P22 protein stained by Calcium binding protein P22 antibody diluted at 1:500. Blue: Hoechst 33342 staining.| Positive Control | HeLa , HepG2 , mouse liver , rat liver |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
