Application
Western blot analysis of Calcium Sensing Receptor in 25 ug of U251 and rat brain lysates. Proteins were transferred to a PVDF membrane and blocked at 4°C overnight. The membrane was probed with Calcium Sensing Receptor antibody at a dilution of 1:500 overnight at 4°C, washed in TBST, and probed with an HRP-conjugated secondary antibody. Chemiluminescent detection was performed.Application
Immunofluorescent analysis of Calcium Sensing Receptor (green) in HeLa cells (right) compared to a negative control without primary antibody (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with Calcium Sensing Receptor antibody in 3% BSA-PBS at a dilution of 1:20 and incubated overnight at 4°C in a humidified chamber. Cells were washed with PBST and incubated with a proper secondary antibody. F-actin (red) was stained with a flourescent red phalloidin and nuclei (blue) were stained with Hoechst or DAPI. Images were taken at a magnification of 60x.| Product Name | Calcium Sensing Receptor antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal antibody to Calcium Sensing Receptora G protein-coupled receptor that senses extracellular calcium; involved in regulating the secretion of parathyroid hormone, bile and intestinal fluid [RGD, Feb 2006] |
| Immunogen | Synthetic peptide corresponding to residues A(12) L A W H S S A Y G P D Q R A Q(27) of rat CaSR. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHCWB |
| ICC/IF: 1:10 - 1:100: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | calcium-sensing receptor Casr |
|---|---|
| Target | Calcium Sensing Receptor |
Application
Western blot analysis of Calcium Sensing Receptor in 25 ug of U251 and rat brain lysates. Proteins were transferred to a PVDF membrane and blocked at 4°C overnight. The membrane was probed with Calcium Sensing Receptor antibody at a dilution of 1:500 overnight at 4°C, washed in TBST, and probed with an HRP-conjugated secondary antibody. Chemiluminescent detection was performed.
Application
Immunofluorescent analysis of Calcium Sensing Receptor (green) in HeLa cells (right) compared to a negative control without primary antibody (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with Calcium Sensing Receptor antibody in 3% BSA-PBS at a dilution of 1:20 and incubated overnight at 4°C in a humidified chamber. Cells were washed with PBST and incubated with a proper secondary antibody. F-actin (red) was stained with a flourescent red phalloidin and nuclei (blue) were stained with Hoechst or DAPI. Images were taken at a magnification of 60x.| Application Notes | ICC/IF: 1:10 - 1:100: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | -20° C, Avoid Freeze/Thaw Cycles |
| Storage Buffer | PBS containing 0.05 % sodium azide |
