Application
Non-transfected (–) and transfected (+) 293T whole cell extracts (15 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Calnexin antibody [GT1563] diluted at 1:6000.Application
Calnexin antibody [GT1563] detects Calnexin protein by Western blot analysis. A. 30 µg 293T whole cell lysate/extract B. 30 µg A431 whole cell lysate/extract C. 30 µg HeLa whole cell lysate/extract D. 30 µg HepG2 whole cell lysate/extract 7.5 % SDS-PAGE Calnexin antibody [GT1563] dilution: 1:1000Application
Calnexin antibody [GT1563] detects Calnexin protein by Western blot analysis. A. 30 µg PC-12 whole cell lysate/extract B. 30 µg Rat-2 whole cell lysate/extract 7.5 % SDS-PAGE Calnexin antibody [GT1563] dilution: 1:500Application
Calnexin antibody [GT1563] detects Calnexin protein by Western blot analysis. A. 50 µg mouse liver lysate/extract 7.5 % SDS-PAGE Calnexin antibody [GT1563] dilution: 1:1000| Product Name | Calnexin antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse Monoclonal antibody [GT1563] to Calnexin (calnexin)This gene encodes a member of the calnexin family of molecular chaperones. The encoded protein is a calcium-binding, endoplasmic reticulum (ER)-associated protein that interacts transiently with newly synthesized N-linked glycoproteins, facilitating protein folding and assembly. It may also play a central role in the quality control of protein folding by retaining incorrectly folded protein subunits within the ER for degradation. Alternatively spliced transcript variants encoding the same protein have been described. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human Calnexin. The exact sequence is proprietary. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2a |
| Clone Number | GT1563 |
| Host Species | Mouse |
| Tested Applications | WB |
| *Optimal dilutions/concentrations should be determined by the researcher.
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| Species Reactivity | HumanMouseRat |
| Predicted Reactivity | Zebrafish, Xenopus laevis, Dog, Pig, Chicken, BovineZebrafish (82%), Xenopus laevis (89%), Dog (94%), Pig (94%), Chicken (91%), Bovine (94%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | P90 CNX IP90 CANX calnexin |
|---|---|
| Target | Calnexin |
| Cellular Localization | Endoplasmic reticulum membrane , Melanosome |
Application
Non-transfected (–) and transfected (+) 293T whole cell extracts (15 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Calnexin antibody [GT1563] diluted at 1:6000.
Application
Calnexin antibody [GT1563] detects Calnexin protein by Western blot analysis. A. 30 µg 293T whole cell lysate/extract B. 30 µg A431 whole cell lysate/extract C. 30 µg HeLa whole cell lysate/extract D. 30 µg HepG2 whole cell lysate/extract 7.5 % SDS-PAGE Calnexin antibody [GT1563] dilution: 1:1000
Application
Calnexin antibody [GT1563] detects Calnexin protein by Western blot analysis. A. 30 µg PC-12 whole cell lysate/extract B. 30 µg Rat-2 whole cell lysate/extract 7.5 % SDS-PAGE Calnexin antibody [GT1563] dilution: 1:500
Application
Calnexin antibody [GT1563] detects Calnexin protein by Western blot analysis. A. 50 µg mouse liver lysate/extract 7.5 % SDS-PAGE Calnexin antibody [GT1563] dilution: 1:1000| Positive Control | mouse liver , PC-12 , Rat2 , 293T , A431 , HeLa , HepG2 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | PBS |
