Application
Various whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Calnexin antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
Calnexin antibody [N3C2], Internal detects Calnexin protein at cytoplasm on mouse duodenum by immunohistochemical analysis. Sample: Paraffin-embedded mouse duodenum. Calnexin antibody [N3C2], Internal diluted at 1:500.Application
Calnexin antibody [N3C2], Internal detects Calnexin protein at endoplasmic reticulum by immunofluorescent analysis. Sample: HeLa cells were fixed in ice-cold MeOH for 5 min. Green: Calnexin protein stained by Calnexin antibody [N3C2], Internal diluted at 1:200. Blue: Hoechst 33342 staining.Application
Sample (30 µg of whole cell lysate) A: 293T B: A431 C: H1299 D: HeLa S3 E: HepG2 F: Molt-4 7.5% SDS PAGE diluted at 1:5000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
Sample (30 µg of whole cell lysate) A:NIH-3T3 7.5% SDS PAGE diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
Immunohistochemical analysis of paraffin-embedded SAS xenograft, using Calnexin antibody at 1:100 dilution.Application
Non-transfected (–) and transfected (+) 293T whole cell extracts (15 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Calnexin antibody [N3C2], Internal diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.| Product Name | Calnexin antibody , Internal |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to Calnexin (calnexin)This gene encodes a member of the calnexin family of molecular chaperones. The encoded protein is a calcium-binding, endoplasmic reticulum (ER)-associated protein that interacts transiently with newly synthesized N-linked glycoproteins, facilitating protein folding and assembly. It may also play a central role in the quality control of protein folding by retaining incorrectly folded protein subunits within the ER for degradation. Alternatively spliced transcript variants encoding the same protein have been described. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human Calnexin. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | HumanMouseRat |
| Predicted Reactivity | Zebrafish, Xenopus laevis, Dog, Pig, Chicken, BovineZebrafish (82%), Xenopus laevis (89%), Dog (94%), Pig (94%), Chicken (91%), Bovine (94%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | CNX antibody FLJ26570 antibody IP90 antibody P90 antibody CANX antibody major histocompatibility complex class I antigen-binding protein p88 antibody calnexin antibody |
|---|---|
| Molecular Weight(MW) | 68 kDa (note) (2) |
| Cellular Localization | Endoplasmic reticulum membrane , Melanosome |
Application
Various whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Calnexin antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
Calnexin antibody [N3C2], Internal detects Calnexin protein at cytoplasm on mouse duodenum by immunohistochemical analysis. Sample: Paraffin-embedded mouse duodenum. Calnexin antibody [N3C2], Internal diluted at 1:500.
Application
Calnexin antibody [N3C2], Internal detects Calnexin protein at endoplasmic reticulum by immunofluorescent analysis. Sample: HeLa cells were fixed in ice-cold MeOH for 5 min. Green: Calnexin protein stained by Calnexin antibody [N3C2], Internal diluted at 1:200. Blue: Hoechst 33342 staining.
Application
Sample (30 µg of whole cell lysate) A: 293T B: A431 C: H1299 D: HeLa S3 E: HepG2 F: Molt-4 7.5% SDS PAGE diluted at 1:5000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
Sample (30 µg of whole cell lysate) A:NIH-3T3 7.5% SDS PAGE diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
Immunohistochemical analysis of paraffin-embedded SAS xenograft, using Calnexin antibody at 1:100 dilution.
Application
Non-transfected (–) and transfected (+) 293T whole cell extracts (15 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Calnexin antibody [N3C2], Internal diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.| Positive Control | 293T , A431 , H1299 , HeLaS3 , HepG2 , Molt-4 , Raji , NIH-3T3 , PC-12 , Rat2 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
