Application
Sample (30 ug of whole cell lysate) A: NT2D1 B: Hela 7.5% SDS PAGE diluted at 1:5000Application
Confocal immunofluorescence analysis (Olympus FV10i) of methanol-fixed HeLa, using Calpain 11 antibody (Green) at 1:200 dilution. Alpha-tubulin filaments were labeled with (Red) at 1:2000.Application
Immunohistochemical analysis of paraffin-embedded SCM1 xenograft, using Calpain 11 antibody at 1:500 dilution.| Product Name | Calpain 11 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to Calpain 11 (calpain 11)Calpains constitute a family of intracellular calcium-dependent cysteine proteases. There are eight members in this superfamily. They consist of a variable 80 kDa subunit and an invariant 30 kDa subunit. This calpain protein appears to have protease activity and calcium-binding ability. A similar mouse protein may play a functional role in spermatogenesis and in the regulation of calcium-dependent signal transduction events during meiosis. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human Calpain 11. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | Human |
| Predicted Reactivity | Mouse, ChimpanzeeMouse (81%), Chimpanzee (98%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | calpain11 antibody CAPN11 antibody calcium-activated neutral proteinase 11 antibody CANP 11 antibody calpain-11 antibody calpain 11 antibody |
|---|---|
| Molecular Weight(MW) | 84 kDa (note) (2) |
| Cellular Localization | Acrosome |
Application
Sample (30 ug of whole cell lysate) A: NT2D1 B: Hela 7.5% SDS PAGE diluted at 1:5000
Application
Confocal immunofluorescence analysis (Olympus FV10i) of methanol-fixed HeLa, using Calpain 11 antibody (Green) at 1:200 dilution. Alpha-tubulin filaments were labeled with (Red) at 1:2000.
Application
Immunohistochemical analysis of paraffin-embedded SCM1 xenograft, using Calpain 11 antibody at 1:500 dilution.| Positive Control | NT2D1 , HeLa |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
