Rabbit Polyclonal antibody to Calpain 12The calpains, calcium-activated neutral proteases, are nonlysosomal, intracellular cysteine proteases. The mammalian calpains include ubiquitous, stomach-specific, and muscle-specific proteins. The ubiquitous enzymes consist of heterodimers with distinct large, catalytic subunits associated with a common small, regulatory subunit. This gene encodes a member of the calpain large subunit family. [provided by RefSeq, Jun 2012]
Immunogen
KLH-conjugated synthetic peptide encompassing a sequence within the center region of human Calpain 12. The exact sequence is proprietary.
Key Feature
Clonality
Polyclonal
Isotype
IgG
Host Species
Rabbit
Tested Applications
IPWB
IP:1:100~1:500 WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher.
Species Reactivity
HumanMonkey
Concentration
1mg/ml
Purification
Affinity purified
Target Information
Gene Synonyms
CAPN12 calpain 12
Target
Calpain 12
Tissue Specificity
Recognizes endogenous levels of Calpain 12 protein.
Application
Application
Western blot analysis of Calpain 12 expression in HepG2 (A); A549 (B) whole cell lysates.
Application Notes
IP:1:100~1:500 WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher.
Additional Information
Form
Liquid
Storage Instructions
Shipped at 4 C. Upon delivery aliquot and store at -20 C for one year. Avoid freeze/thaw cycles.
Storage Buffer
Liquid in 0.42% Potassium phosphate; 0.87% Sodium chloride; pH 7.3; 30% glycerol; and 0.01% sodium azide.