Application
Immunohistochemistry was performed on cancer biopsies of deparaffinized human breast carcinoma tissues. To expose target proteins, heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1:200 with or without Calpastatin antibody [1F7E3D10] overnight at 4°C in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP, followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.| Product Name | Calpastatin antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal antibody [1F7E3D10] to Calpastatin |
| Immunogen | Purified bovine skeletal muscle 80 kDa subunit of m-Calpastatin. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | 1F7E3D10 |
| Host Species | Mouse |
| Tested Applications | ICC/IFIHCIHC-PWB |
| ICC/IF: 1:100 - 1:200: |
|
| Species Reactivity | HumanPigRatFishBovine |
| Concentration | 1mg/ml |
| Gene Synonyms | calpastatin - CAST |
|---|---|
| Target | Calpastatin |
| Tissue Specificity | This antibody does not cross-react with calpains or calmodulin. |
Application
Immunohistochemistry was performed on cancer biopsies of deparaffinized human breast carcinoma tissues. To expose target proteins, heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1:200 with or without Calpastatin antibody [1F7E3D10] overnight at 4°C in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP, followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.| Application Notes | ICC/IF: 1:100 - 1:200: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | -20° C, Avoid Freeze/Thaw Cycles |
| Storage Buffer | Ascites containing 0.05 % sodium azide |
