Application
IHC image using - Detection of Calponin by IHC in human breast| Product Name | Calponin antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal [CALP] to CalponinCalponin, a calmodulin, binds tropomyosin and F actin and is thought to be involved in the regulation of smooth muscle contraction. The expression of calponin is restricted to smooth muscle cells and is a marker of the differentiated contractile phenotype of developing smooth muscle. Two isoforms of calponin exist whose molecular weights are 34 kDa and 29 kDa. Expression of the 29 kDa form is primarily restricted to muscle of the urogenital tract. The expression of calponin has also been demonstrated in myoepithelial cells from benign and malignant breast lesions. |
| Immunogen | Crude human uterus extract |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | CALP |
| Host Species | Mouse |
| Tested Applications | IHC-P |
| This antibody may be diluted to a titer of 1:50-1:100 in an ABC method. However, depending on the staining conditions employed, we suggest that final dilution should be determined by the user. We suggest an incubation period of 30 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation should be determined by the end user. Formalin fixed paraffin embedded tissue sections require digestion of tissue sections with trypsin and followed by high temperature antigen unmasking with 10 mM citrate buffer, pH 6.0 prior to immunostaining.: |
|
| Species Reactivity | HumanRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | P51911 1264 Sm-Calp Calponin SmCalp 600806 SMCC CNN1 Sm Calp |
|---|---|
| Target | Calponin |
| Tissue Specificity | The antibody does not cross-react with skeletal, cardiac, or non-muscle tissue calponin. |
| Cellular Localization | Cytoplasmic. |
| Light Chain Type | kappa |
Application
IHC image using - Detection of Calponin by IHC in human breast| Positive Control | Myoepithelium in normal breast glands |
|---|---|
| Application Notes | This antibody may be diluted to a titer of 1:50-1:100 in an ABC method. However, depending on the staining conditions employed, we suggest that final dilution should be determined by the user. We suggest an incubation period of 30 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation should be determined by the end user. Formalin fixed paraffin embedded tissue sections require digestion of tissue sections with trypsin and followed by high temperature antigen unmasking with 10 mM citrate buffer, pH 6.0 prior to immunostaining.: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution, aliquot and store at 4°C. |
| Storage Buffer | Preservative: sodium azide |
