Application
Western blot analysis of CaMK2 phosphorylation expression in K562 whole cell lysates,The lane on the left is treated with the antigen-specific peptide.| Product Name | CaMKII alpha (phospho Thr286) antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to CaMKII alpha (phospho Thr286)The product of this gene belongs to the serine/threonine protein kinases family, and to the Ca(2+)/calmodulin-dependent protein kinases subfamily. Calcium signaling is crucial for several aspects of plasticity at glutamatergic synapses. This calcium calmodulin-dependent protein kinase is composed of four different chains: alpha, beta, gamma, and delta. The alpha chain encoded by this gene is required for hippocampal long-term potentiation (LTP) and spatial learning. In addition to its calcium-calmodulin (CaM)-dependent activity, this protein can undergo autophosphorylation, resulting in CaM-independent activity. Two transcript variants encoding distinct isoforms have been identified for this gene. [provided by RefSeq, Nov 2008] |
| Immunogen | A synthesized peptide derived from human CaMK2 around the phosphorylation site of Thr286 |
| Modification | p-Thr286 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHCWB |
| WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500: |
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| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | phospho CaMK2 (phospho Thr286) (pT286) Phospho Thr286 CaMK2 (phospho Thr286) phospho CaMK2 (phospho Thr286) (pThr286) Phospho CaMK2 (phospho Thr286) Thr286 CaMK2 (phospho Thr286) Phospho T286 calcium/calmo |
|---|---|
| Target | CaMK2 (phospho Thr286) |
| Tissue Specificity | CAMK2D |
Application
Western blot analysis of CaMK2 phosphorylation expression in K562 whole cell lysates,The lane on the left is treated with the antigen-specific peptide.| Application Notes | WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500: |
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| Form | Liquid |
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| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20oC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
