Rabbit Polyclonal antibody to CaMKII alphaCaM-kinase II (CAMK2) is a prominent kinase in the central nervous system that may function in long-term potentiation and neurotransmitter release. Member of the NMDAR signaling complex in excitatory synapses it may regulate NMDAR-dependent potentiation of the AMPAR and synaptic plasticity
Immunogen
Peptide sequence around aa.284~288 (Q-E-T-V-D) derived from Human CaMKII.
Key Feature
Clonality
Polyclonal
Host Species
Rabbit
Tested Applications
ICC/IFWB
Suggested starting dilutions are as follows. WB: 1:500~1:1000, ICC/IF: 1:100~1:200. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.:
Species Reactivity
HumanMouseRat
Concentration
1mg/ml (Please refer to the vial label for the specific concentration)
Purification
Affinity purified
Target Information
Target
CaMKII
Molecular Weight(MW)
50 (note)
Application
Application
Western blot analysis of extracts from Hela using CaMKII Antibody .
Application
Immunofluorescence staining of paraffin-embedded human hippocampal region tissue from the falling sickness disease using CaMKII Antibody .
Application Notes
Suggested starting dilutions are as follows. WB: 1:500~1:1000, ICC/IF: 1:100~1:200. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.:
Additional Information
Form
Liquid
Storage Instructions
Keep as concentrated solution. Aliquot and store at -20oC or below. Avoid multiple freeze-thaw cycles.
Storage Buffer
saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.