Application
Various whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with CaMKII delta antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
Immunoprecipitation of CaMKII delta protein from HepG2 whole cell extracts using 5 μg of CaMKII delta antibody . Western blot analysis was performed using CaMKII delta antibody . EasyBlot anti-Rabbit IgG was used as a secondary reagent.Application
CaMK2D antibody detects CaMK2D protein by western blot analysis. A. 30 µg A549 whole cell lysate/extract B. 30 µg H1299 whole cell lysate/extract C. 30 µg HCT116 whole cell lysate/extract 10% SDS-PAGE CaMK2D antibody dilution: 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
Various tissue extracts (50 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with CaMKII delta antibody diluted at 1:50000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
CaMKII delta antibody detects CaMKII delta protein at cytoplasm in rat heart by immunohistochemical analysis. Sample: Paraffin-embedded rat heart. CaMKII delta antibody diluted at 1:400.Application
CaMKII delta antibody detects CaMKII delta protein at cytoplasm by immunofluorescent analysis. Sample: HCT116 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: CaMKII delta protein stained by CaMKII delta antibody diluted at 1:500. Blue: Hoechst 33342 staining.Application
CaMKII delta antibody detects CaMKII delta protein at cytoplasm in mouse muscle by immunohistochemical analysis. Sample: Paraffin-embedded mouse muscle. CaMKII delta antibody diluted at 1:400.| Product Name | CaMKII delta antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to CaMKII delta (calcium/calmodulin-dependent protein kinase II delta)The product of this gene belongs to the serine/threonine protein kinase family and to the Ca(2+)/calmodulin-dependent protein kinase subfamily. Calcium signaling is crucial for several aspects of plasticity at glutamatergic synapses. In mammalian cells, the enzyme is composed of four different chains: alpha, beta, gamma, and delta. The product of this gene is a delta chain. Alternative splicing results in multiple transcript variants encoding distinct isoforms. Distinct isoforms of this chain have different expression patterns. |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human CaMKII delta. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IF,IHC-P,IP,WB |
| *Optimal dilutions/concentrations should be determined by the researcher. | |
| Species Reactivity | Human,Mouse,Rat,Rabbit |
| Predicted Reactivity | Xenopus laevis, Pig, Chicken, BovineXenopus laevis (94%), Pig (100%), Chicken (98%), Bovine (99%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | CAMKD antibody, DKFZp686G23119 antibody, DKFZp686I2288 antibody, MGC44911 antibody, CAMK2D antibody, caM kinase II subunit delta antibody, CaM kinase II delta subunit antibody, CaMK-II delta subunit antibody, CaM-kinase II delta chain antibody, calcium/cal |
|---|---|
| Molecular Weight(MW) | 56 kDa (note) (6) |
| Tissue Specificity | Cross-reactivity may occur with other CaM kinase II subunits. |
Application
Various whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with CaMKII delta antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
Immunoprecipitation of CaMKII delta protein from HepG2 whole cell extracts using 5 μg of CaMKII delta antibody . Western blot analysis was performed using CaMKII delta antibody . EasyBlot anti-Rabbit IgG was used as a secondary reagent.
Application
CaMK2D antibody detects CaMK2D protein by western blot analysis. A. 30 µg A549 whole cell lysate/extract B. 30 µg H1299 whole cell lysate/extract C. 30 µg HCT116 whole cell lysate/extract 10% SDS-PAGE CaMK2D antibody dilution: 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
Various tissue extracts (50 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with CaMKII delta antibody diluted at 1:50000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
CaMKII delta antibody detects CaMKII delta protein at cytoplasm in rat heart by immunohistochemical analysis. Sample: Paraffin-embedded rat heart. CaMKII delta antibody diluted at 1:400.
Application
CaMKII delta antibody detects CaMKII delta protein at cytoplasm by immunofluorescent analysis. Sample: HCT116 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: CaMKII delta protein stained by CaMKII delta antibody diluted at 1:500. Blue: Hoechst 33342 staining.
Application
CaMKII delta antibody detects CaMKII delta protein at cytoplasm in mouse muscle by immunohistochemical analysis. Sample: Paraffin-embedded mouse muscle. CaMKII delta antibody diluted at 1:400.| Positive Control | 293T , A431 , HeLa , HepG2 , A549 , H1299 , HCT116 , mouse brain , rat brain |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher. |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 1% BSA, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative. |
