Application
Carbonic Anhydrase IX antibody [GT14212] detects Carbonic Anhydrase IX protein at membrane by immunofluorescent analysis. Sample: 200μM CoCl2 for 48 hr A431 cells were fixed in ice-cold MeOH for 5 min. Green: Carbonic Anhydrase IX protein stained by Carbonic Anhydrase IX antibody [GT14212] diluted at 1:100. Blue: Hoechst 33342 staining.Application
Carbonic Anhydrase IX antibody [GT14212] detects Carbonic Anhydrase IX protein by Western blot analysis. A. 30 µg HeLa whole cell lysate/extract (untreated) B. 30 µg HeLa whole cell lysate/extract (200 µM CoCl2 treatment for 24 hr) C. 30u g HeLa whole cell lysate/extract (200 µM CoCl2 treatment for 48 hr) D. 30 µg HeLa whole cell lysate/extract (untreated) E. 30 µg HeLa whole cell lysate/extract (1% O2 treatment for 24 hr) F. 30 µg HeLa whole cell lysate/extract (1% O2 treatment for 48 hr) 7.5 % SDS-PAGE Carbonic Anhydrase IX antibody [GT14212] dilution: 1:1000| Product Name | Carbonic Anhydrase IX antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse Monoclonal antibody to Carbonic Anhydrase IX (carbonic anhydrase IX)Carbonic anhydrases (CAs) are a large family of zinc metalloenzymes that catalyze the reversible hydration of carbon dioxide. They participate in a variety of biological processes, including respiration, calcification, acid-base balance, bone resorption, and the formation of aqueous humor, cerebrospinal fluid, saliva, and gastric acid. They show extensive diversity in tissue distribution and in their subcellular localization. CA IX is a transmembrane protein and the only tumor-associated carbonic anhydrase isoenzyme known. It is expressed in all clear-cell renal cell carcinoma, but is not detected in normal kidney or most other normal tissues. It may be involved in cell proliferation and transformation. This gene was mapped to 17q21.2 by fluorescence in situ hybridization, however, radiation hybrid mapping localized it to 9p13-p12. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the N-terminus region of human Carbonic Anhydrase IX. The exact sequence is proprietary. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | GT14212 |
| Host Species | Mouse |
| Tested Applications | ICC/IFWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
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| Species Reactivity | Human |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | CAIX carbonic anhydrase IX MN CA9 |
|---|---|
| Target | Carbonic Anhydrase IX |
| Cellular Localization | Nucleus , nucleolus , Cell membrane , Cell projection , microvillus membrane |
Application
Carbonic Anhydrase IX antibody [GT14212] detects Carbonic Anhydrase IX protein at membrane by immunofluorescent analysis. Sample: 200μM CoCl2 for 48 hr A431 cells were fixed in ice-cold MeOH for 5 min. Green: Carbonic Anhydrase IX protein stained by Carbonic Anhydrase IX antibody [GT14212] diluted at 1:100. Blue: Hoechst 33342 staining.
Application
Carbonic Anhydrase IX antibody [GT14212] detects Carbonic Anhydrase IX protein by Western blot analysis. A. 30 µg HeLa whole cell lysate/extract (untreated) B. 30 µg HeLa whole cell lysate/extract (200 µM CoCl2 treatment for 24 hr) C. 30u g HeLa whole cell lysate/extract (200 µM CoCl2 treatment for 48 hr) D. 30 µg HeLa whole cell lysate/extract (untreated) E. 30 µg HeLa whole cell lysate/extract (1% O2 treatment for 24 hr) F. 30 µg HeLa whole cell lysate/extract (1% O2 treatment for 48 hr) 7.5 % SDS-PAGE Carbonic Anhydrase IX antibody [GT14212] dilution: 1:1000| Positive Control | A431 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | PBS |
