Application
Carboxypeptidase E antibody [N2C2], Internal detects Carboxypeptidase E protein at nucleus on human breast cancer by immunohistochemical analysis. Sample: Paraffin-embedded breast cancer. Carboxypeptidase E antibody [N2C2], Internal dilution: 1:500.Application
Sample (20 µg of whole cell lysate) A: mouse brain 10% SDS PAGE diluted at 1:10000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
Western Blot analysis of CPE expression in transfected 293T cell line by CPE polyclonal antibody. A: Non-transfected lysate. B: CPE transfected lysate. 12% SDS PAGE diluted at 1:500 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.| Product Name | Carboxypeptidase E antibody , Internal |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to Carboxypeptidase E (carboxypeptidase E)This gene encodes a carboxypeptidase that cleaves C-terminal amino acid residues and is involved in the biosynthesis of peptide hormones and neurotransmitters, including insulin. It is a peripheral membrane protein. The protein specifically binds regulated secretory pathway proteins, including prohormones, but not constitutively secreted proteins. Mutations in this gene are implicated in type II diabetes. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human Carboxypeptidase E. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
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| Species Reactivity | HumanMouse |
| Predicted Reactivity | Rat, Zebrafish, Xenopus laevis, Pig, Rhesus Monkey, Chimpanzee, BovineRat (96%), Zebrafish (85%), Xenopus laevis (83%), Pig (95%), Rhesus Monkey (99%), Chimpanzee (99%), Bovine (94%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | CPE antibody carboxypeptidase E antibody enkephalin convertase antibody CPH antibody carboxypeptidase H antibody prohormone-processing carboxypeptidase antibody |
|---|---|
| Molecular Weight(MW) | 53 kDa (note) |
| Cellular Localization | Secreted |
Application
Carboxypeptidase E antibody [N2C2], Internal detects Carboxypeptidase E protein at nucleus on human breast cancer by immunohistochemical analysis. Sample: Paraffin-embedded breast cancer. Carboxypeptidase E antibody [N2C2], Internal dilution: 1:500.
Application
Sample (20 µg of whole cell lysate) A: mouse brain 10% SDS PAGE diluted at 1:10000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
Western Blot analysis of CPE expression in transfected 293T cell line by CPE polyclonal antibody. A: Non-transfected lysate. B: CPE transfected lysate. 12% SDS PAGE diluted at 1:500 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.| Positive Control | Carboxypeptidase H tansfected 293T cell , mouse brain |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
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| Form | Liquid |
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| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
