Application
Sample(30 ug whole cell lysate) A:Hep G2 7.5% SDS PAGE diluted at 1:500Application
Carboxypeptidase M antibody [N1C2] detects Carboxypeptidase M protein by western blot analysis. Rat tissue extracts (50 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with Carboxypeptidase M antibody [N1C2] diluted at 1:500.| Product Name | Carboxypeptidase M antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal antibody to Carboxypeptidase M (carboxypeptidase M)The protein encoded by this gene is a membrane-bound arginine/lysine carboxypeptidase. Its expression is associated with monocyte to macrophage differentiation. This encoded protein contains hydrophobic regions at the amino and carboxy termini and has 6 potential asparagine-linked glycosylation sites. The active site residues of carboxypeptidases A and B are conserved in this protein. Three alternatively spliced transcript variants encoding the same protein have been described for this gene. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human Carboxypeptidase M. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| *Optimal dilutions/concentrations should be determined by the researcher.
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| Species Reactivity | HumanRat |
| Predicted Reactivity | MouseMouse (87%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | CPM antibody carboxypeptidase M antibody |
|---|---|
| Target | Carboxypeptidase M |
| Molecular Weight(MW) | 51 kDa (note) (3) |
| Cellular Localization | Cell membrane , GPI-anchor |
Application
Sample(30 ug whole cell lysate) A:Hep G2 7.5% SDS PAGE diluted at 1:500
Application
Carboxypeptidase M antibody [N1C2] detects Carboxypeptidase M protein by western blot analysis. Rat tissue extracts (50 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with Carboxypeptidase M antibody [N1C2] diluted at 1:500.| Positive Control | HepG2 , Rat liver |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
