Application
Carboxypeptidase N1 antibody detects Carboxypeptidase N1 protein by western blot analysis. Whole cell extracts (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with Carboxypeptidase N1 antibody diluted at 1:500.| Product Name | carboxypeptidase N1 antibody |
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| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to carboxypeptidase N1 (carboxypeptidase N, polypeptide 1)Carboxypeptidase N is a plasma metallo-protease that cleaves basic amino acids from the C terminal of peptides and proteins. The enzyme is important in the regulation of peptides like kinins and anaphylatoxins, and has also been known as kininase-1 and anaphylatoxin inactivator. This enzyme is a tetramer comprised of two identical regulatory subunits and two identical catalytic subunits; this gene encodes the catalytic subunit. Mutations in this gene can be associated with angioedema or chronic urticaria resulting from carboxypeptidase N deficiency. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human carboxypeptidase N1. The exact sequence is proprietary. |
| Clonality | Polyclonal |
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| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| *Optimal dilutions/concentrations should be determined by the researcher.
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| Species Reactivity | Human |
| Predicted Reactivity | Mouse, Rat, BovineMouse (87%), Rat (86%), Bovine (89%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | CPN antibody FLJ40792 antibody SCPN antibody CPN1 antibody carboxypeptidase N polypeptide 1 50 kD antibody arginine carboxypeptidase antibody carboxypeptidase N catalytic chain antibody serum carboxypeptidase N antibody carboxypeptidase N catalytic |
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| Molecular Weight(MW) | 52 kDa (note) |
| Cellular Localization | Secreted , extracellular space |
Application
Carboxypeptidase N1 antibody detects Carboxypeptidase N1 protein by western blot analysis. Whole cell extracts (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with Carboxypeptidase N1 antibody diluted at 1:500.| Positive Control | HepG2 conditioned medium |
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| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
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| Form | Liquid |
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| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
