Application
Sample (30 µg of whole cell lysate) A: 293T 7.5% SDS PAGE diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
CARD10 antibody [N2C1], Internal detects CARD10 protein at cytoplasm on human breast carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human breast carcinoma. CARD10 antibody [N2C1], Internal diluted at 1:250.| Product Name | CARD10 antibody , Internal |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal antibody to CARD10 (caspase recruitment domain family, member 10)The caspase recruitment domain (CARD) is a protein module that consists of 6 or 7 antiparallel alpha helices. It participates in apoptosis signaling through highly specific protein-protein homophilic interactions. Like several other CARD proteins, CARD10 belongs to the membrane-associated guanylate kinase (MAGUK) family and activates NF-kappa-B (NFKB; see MIM 164011) through BCL10 (MIM 603517) (Wang et al., 2001 [PubMed 11259443]).[supplied by OMIM] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human CARD10. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHC-FIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
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| Species Reactivity | HumanMouse |
| Predicted Reactivity | RatRat (93%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | BIMP1 antibody CARMA3 antibody MGC142219 antibody CARD10 antibody Bcl10 binding protein and activator of NFKB antibody CARD-containing MAGUK protein 3 antibody CARD-containing MAGUK 3 protein antibody caspase recruitment domain-containing protein 10 |
|---|---|
| Target | CARD10 |
| Molecular Weight(MW) | 116 kDa (note) |
| Cellular Localization | Cytoplasm |
Application
Sample (30 µg of whole cell lysate) A: 293T 7.5% SDS PAGE diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
CARD10 antibody [N2C1], Internal detects CARD10 protein at cytoplasm on human breast carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human breast carcinoma. CARD10 antibody [N2C1], Internal diluted at 1:250.| Positive Control | 293T , H1299 , Molt-4 , Raji |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
