Application
CARD11 antibody [N1N2], N-term detects CARD11 protein by western blot analysis. A. 30 µg Raji whole cell lysate/extract B. 30 µg K562 whole cell lysate/extract C. 30 µg NCI-H929 whole cell lysate/extract 5% SDS-PAGE CARD11 antibody [N1N2], N-term dilution: 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
Immunohistochemical analysis of paraffin-embedded SW480 xenograft, using CARD11 antibody at 1:100 dilution.Application
CARD11 antibody [N1N2], N-term detects CARD11 protein by western blot analysis. A. 30 µg BCL-1 whole cell lysate/extract 5% SDS-PAGE CARD11 antibody [N1N2], N-term dilution: 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.| Product Name | CARD11 antibody , N-term |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to CARD11 (caspase recruitment domain family, member 11)The protein encoded by this gene belongs to the membrane-associated guanylate kinase (MAGUK) family, a class of proteins that functions as molecular scaffolds for the assembly of multiprotein complexes at specialized regions of the plasma membrane. This protein is also a member of the CARD protein family, which is defined by carrying a characteristic caspase-associated recruitment domain (CARD). This protein has a domain structure similar to that of CARD14 protein. The CARD domains of both proteins have been shown to specifically interact with BCL10, a protein known to function as a positive regulator of cell apoptosis and NF-kappaB activation. When expressed in cells, this protein activated NF-kappaB and induced the phosphorylation of BCL10. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the N-terminus region of human CARD11. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
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| Species Reactivity | HumanMouse |
| Predicted Reactivity | Chicken, BovineChicken (87%), Bovine (98%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | BIMP3 antibody CARMA1 antibody MGC133069 antibody CARD11 antibody CARD-containing MAGUK protein 1 antibody card-maguk protein 1 antibody caspase recruitment domain-containing protein 11 antibody carma 1 antibody bcl10-interacting maguk protein 3 an |
|---|---|
| Molecular Weight(MW) | 133 kDa (note) (3) |
| Cellular Localization | Cytoplasm |
Application
CARD11 antibody [N1N2], N-term detects CARD11 protein by western blot analysis. A. 30 µg Raji whole cell lysate/extract B. 30 µg K562 whole cell lysate/extract C. 30 µg NCI-H929 whole cell lysate/extract 5% SDS-PAGE CARD11 antibody [N1N2], N-term dilution: 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
Immunohistochemical analysis of paraffin-embedded SW480 xenograft, using CARD11 antibody at 1:100 dilution.
Application
CARD11 antibody [N1N2], N-term detects CARD11 protein by western blot analysis. A. 30 µg BCL-1 whole cell lysate/extract 5% SDS-PAGE CARD11 antibody [N1N2], N-term dilution: 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.| Positive Control | Raji , K562 , NCI-H929 , BCL-1 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
