Application
Sample (30 ug of whole cell lysate) A: MCF-7 5% SDS PAGE diluted at 1:1000Application
CARD12 antibody detects CARD12 protein by western blot analysis. Non-transfected (-) and CARD12 -transfected (+, ) 293T whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with CARD12 antibody diluted by 1:5000.| Product Name | CARD12 antibody , N-term |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to CARD12 (NLR family, CARD domain containing 4)In C. elegans, Ced4 binds and activates Ced3, an apoptotic initiator caspase, via caspase-associated recruitment domains (CARDs). Human Ced4 homologs include APAF1 (MIM 602233), NOD1/CARD4 (MIM 605980), and NOD2/CARD15 (MIM 605956). These proteins have at least 1 N-terminal CARD domain followed by a centrally located nucleotide-binding domain (NBD or NACHT) and a C-terminal regulatory domain, found only in mammals, that contains either WD40 repeats or leucine-rich repeats (LRRs). CARD12 is a member of the Ced4 family and can induce apoptosis.[supplied by OMIM] |
| Immunogen | Recombinant protein encompassing a sequence within the N-terminus region of human CARD12. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | Human |
| Predicted Reactivity | BovineBovine (82%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | CARD12 antibody CLAN antibody CLAN1 antibody CLANA antibody CLANB antibody CLANC antibody CLAND antibody CLR2.1 antibody IPAF antibody NLRC4 antibody NLR family CARD domain-containing protein 4 antibody ICE-protease activating factor antibody c |
|---|---|
| Molecular Weight(MW) | 116 kDa (note) (3) |
| Cellular Localization | Cytoplasm |
Application
Sample (30 ug of whole cell lysate) A: MCF-7 5% SDS PAGE diluted at 1:1000
Application
CARD12 antibody detects CARD12 protein by western blot analysis. Non-transfected (-) and CARD12 -transfected (+, ) 293T whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with CARD12 antibody diluted by 1:5000.| Positive Control | MCF-7 , human NLRC4-transfected 293T |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
