Application
Sample(30 μg of whole cell lysate) A:A431 B:Hep G2 10% SDS PAGE diluted at 1:500Application
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using CARD8 antibody at 1:200 dilution.| Product Name | CARD8 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal antibody to CARD8 (caspase recruitment domain family, member 8)The protein encoded by this gene belongs to the caspase recruitment domain (CARD)-containing family of proteins, which are involved in pathways leading to activation of caspases or nuclear factor kappa-B (NFKB). This protein may be a component of the inflammasome, a protein complex that plays a role in the activation of proinflammatory caspases. It is thought that this protein acts as an adaptor molecule that negatively regulates NFKB activation, CASP1-dependent IL1B secretion, and apoptosis. Polymorphisms in this gene may be associated with a susceptibility to rheumatoid arthritis. Alternatively spliced transcript variants have been described for this gene, but their biological validity has not been determined. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the C-terminus region of human CARD8. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | CARDINAL antibody DACAR antibody DAKAR antibody DKFZp779L0366 antibody FLJ18119 antibody FLJ18121 antibody KIAA0955 antibody MGC57162 antibody NDPP antibody NDPP1 antibody TUCAN antibody CARD8 antibody apoptotic protein NDPP1 antibody caspase |
|---|---|
| Target | CARD8 |
| Molecular Weight(MW) | 49 kDa (note) (3) |
| Cellular Localization | Cytoplasm , Nucleus |
Application
Sample(30 μg of whole cell lysate) A:A431 B:Hep G2 10% SDS PAGE diluted at 1:500
Application
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using CARD8 antibody at 1:200 dilution.| Positive Control | 293T , A431 , HepG2 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 40% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
