Recognition of the dephospho- and phospho- forms of human cardiac troponin I. A. Cardiac Troponin I antibody [10B11] ; B. Cardiac Troponin I antibody [8E10]
Mouse monoclonal [10B11] to cardiac Troponin ITroponin I is the inhibitory subunit of troponin, the thin filament regulatory complex which confers calcium-sensitivity to striated muscle actomyosin ATPase activity. It also binds to actin and tropomyosin.
Immunogen
epitope:16 – 20 a.a of human cardiac Troponin I
Key Feature
Clonality
Monoclonal
Isotype
IgG1
Clone Number
10B11
Host Species
Mouse
Tested Applications
PurifyIAWB
WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher.
Reacts with free cardiac troponin I (cTnI) and cTnI forming complexes with other troponin components (in the presence of 5 mM EDTA). Not affected by heparin, phosphorylation, oxidation and troponin complex formation. Recognizes dephospho- and phospho- forms of human cardiac Troponin I.Does not cross-react with skeletal muscle troponin I.
Application
Application
Recognition of the dephospho- and phospho- forms of human cardiac troponin I. A. Cardiac Troponin I antibody [10B11] ; B. Cardiac Troponin I antibody [8E10]
Application Notes
WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher.
Additional Information
Form
Liquid
Storage Instructions
For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.