Application
Cardiac troponin I measurements using sandwich immunoassays to determine the total cardiac troponin I in serial blood samples from AMI patient.Application
Detection of cardiac troponin I (cTnI) by Western blotting. After SDS-PAGE cTnI was transferred to a nitrocellulose membrane and probed by Cardiac Troponin I antibody [16A11] and then by a HRP-conjugated goat anti-mouse IgG antibody. Substrate - diaminobenzidine. Lane 1: 3 ng cTnI; Lane 2: 10 ng cTnI; Lane 3: 30 ng cTnI; Lane 4: 100 ng cTnI.| Product Name | Cardiac Troponin I antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal [16A11] to cardiac Troponin ITroponin I is the inhibitory subunit of troponin, the thin filament regulatory complex which confers calcium-sensitivity to striated muscle actomyosin ATPase activity. It also binds to actin and tropomyosin. |
| Immunogen | epitope:86 – 90 a.a of human cardiac Troponin I |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | 16A11 |
| Host Species | Mouse |
| Tested Applications | PurifyELISAIAWB |
| This antibody is recommend for better sensitivity in WB. Recommended pair for ELISA: clone 19C7 (GTX28289) for capture and clone 16A11 (this antibody) for detection.: |
|
| Species Reactivity | HumanMouseDogPigRatCatGoatRabbitBovine |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Synonyms | 7137 CMH7 TNNI3 Cardiac Troponin I cTnI 115210 P19429 TNNC1 MGC116817 |
|---|---|
| Target | Cardiac Troponin I |
| Tissue Specificity | reacts with free cardiac troponin I (cTnI) and cTnI forming complexes with other troponin components (in the presence of 5 mM EDTA). Not affected by heparin, phosphorylation, oxidation and troponin complex formation. Does not cross-react with skeletal muscle troponin I. |
Application
Cardiac troponin I measurements using sandwich immunoassays to determine the total cardiac troponin I in serial blood samples from AMI patient.
Application
Detection of cardiac troponin I (cTnI) by Western blotting. After SDS-PAGE cTnI was transferred to a nitrocellulose membrane and probed by Cardiac Troponin I antibody [16A11] and then by a HRP-conjugated goat anti-mouse IgG antibody. Substrate - diaminobenzidine. Lane 1: 3 ng cTnI; Lane 2: 10 ng cTnI; Lane 3: 30 ng cTnI; Lane 4: 100 ng cTnI.| Application Notes | This antibody is recommend for better sensitivity in WB. Recommended pair for ELISA: clone 19C7 (GTX28289) for capture and clone 16A11 (this antibody) for detection.: |
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| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4 degrees Celcius. |
| Storage Buffer | Preservative: 0.1% Sodium Azide; Constituents: PBS. pH 7.4 |
