| Product Name | Cardiac Troponin I antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal [2D5] to Cardiac Troponin IMonoclonal Mouse Anti-Human Cardiac Troponin I |
| Immunogen | Purified human cardiac troponin I |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | 2D5 |
| Host Species | Mouse |
| Tested Applications | IHC-FIHC-P |
| Immunohistochemistry 1:25-1:50.( For formalin-fixed paraffin-embedded (FFPE) tissue sections antigen demasker (10 mM citrate buffer pH 6.0 ) is requied. Boil the tissue 10-20 mins in this buffer, followed by cooling to RT. This antibody can be used on formalin-fixed, paraffin-embedded tissue sections. Prolonged fixation in buffered formalin can destroy the epitope. The antibody may be used at a dilution of 1:25-1:50 with LAB/Probe. When using paraffin-embedded and formalin-fixed tissues, high temperature antigenic unmasking technique (see instructions below) is strongly recommended for consistent and reproducible results. This product can also be used on frozen tissue sections. The optimal conditions should be determined by the individual laboratory.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | 7137 CMH7 TNNI3 Cardiac Troponin I cTnI 115210 P19429 TNNC1 MGC116817 |
|---|---|
| Target | Cardiac Troponin I |
| Tissue Specificity | This antibody is specific for human cardiac troponin subunit I, a 21 kD protein responsible for inhibiting actomyosin Mg2+-ATPase activity. |
| Application Notes | Immunohistochemistry 1:25-1:50.( For formalin-fixed paraffin-embedded (FFPE) tissue sections antigen demasker (10 mM citrate buffer pH 6.0 ) is requied. Boil the tissue 10-20 mins in this buffer, followed by cooling to RT. This antibody can be used on formalin-fixed, paraffin-embedded tissue sections. Prolonged fixation in buffered formalin can destroy the epitope. The antibody may be used at a dilution of 1:25-1:50 with LAB/Probe. When using paraffin-embedded and formalin-fixed tissues, high temperature antigenic unmasking technique (see instructions below) is strongly recommended for consistent and reproducible results. This product can also be used on frozen tissue sections. The optimal conditions should be determined by the individual laboratory.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution, aliquot and store at 4°C. Do not freeze. |
| Storage Buffer | Phosphate-buffered saline, pH 7.2, containing 0.2% BSA and 0.09% sodium azide |
