Application
Immunohistochemical staining of paraffin-embedded prostate tissue using anti-TNNI3mouse monoclonal antibody. ( Dilution 1:50)Application
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TNNI3 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TNNI3.| Product Name | Cardiac Troponin I antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal antibody [3G2] to Cardiac Troponin ITroponin I is the inhibitory subunit of troponin, the thin filament regulatory complex which confers calcium-sensitivity to striated muscle actomyosin ATPase activity. |
| Immunogen | Recombinant protein expressed in E.coli corresponding to amino acids 1-210 of human TNNI3 |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2a |
| Clone Number | 3G2 |
| Host Species | Mouse |
| Tested Applications | IHCIHC-PWB |
| Optimal working dilutions should be determined experimentally by the end user.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | CMH7 cTnI TNNI3 TNNC1 MGC116817 |
|---|---|
| Target | Cardiac Troponin I |
| Tissue Specificity | is specific for amino acids 1-210 of human TNNI3 |
Application
Immunohistochemical staining of paraffin-embedded prostate tissue using anti-TNNI3mouse monoclonal antibody. ( Dilution 1:50)
Application
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TNNI3 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TNNI3.| Application Notes | Optimal working dilutions should be determined experimentally by the end user.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4°C or aliquot into working amounts and store at -20°C. For long-term storage, store at -70°C (aliquotted). Avoid repeated freeze-thaw cycles. |
| Storage Buffer | PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide. |
