Application
Immunofluorescent staining of A549 cells using anti-TNNI3 mouse monoclonal antibody .Application
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-TNNI3 monoclonal antibody.Application
Anti-TNNI3 mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected with TNNI3Application
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TNNI3 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TNNI3.Application
Immunofluorescent staining of HeLa cells using anti-TNNI3 mouse monoclonal antibody .| Product Name | Cardiac Troponin I antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal antibody [4E5] to Cardiac Troponin ITroponin I is the inhibitory subunit of troponin, the thin filament regulatory complex which confers calcium-sensitivity to striated muscle actomyosin ATPase activity. |
| Immunogen | Recombinant protein expressed in E.coli corresponding to amino acids 1-210 of human TNNI3 |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | 4E5 |
| Host Species | Mouse |
| Tested Applications | ICC/IFWB |
| Optimal working dilutions should be determined experimentally by the end user.: |
|
| Species Reactivity | HumanDogMonkeyRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | CMH7 cTnI TNNI3 TNNC1 MGC116817 |
|---|---|
| Target | Cardiac Troponin I |
| Tissue Specificity | is specific for amino acids 1-210 of human TNNI3 |
Application
Immunofluorescent staining of A549 cells using anti-TNNI3 mouse monoclonal antibody .
Application
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-TNNI3 monoclonal antibody.
Application
Anti-TNNI3 mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected with TNNI3
Application
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TNNI3 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TNNI3.
Application
Immunofluorescent staining of HeLa cells using anti-TNNI3 mouse monoclonal antibody .| Application Notes | Optimal working dilutions should be determined experimentally by the end user.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4°C or aliquot into working amounts and store at -20°C. For long-term storage, store at -70°C (aliquotted). Avoid repeated freeze-thaw cycles. |
| Storage Buffer | PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide. |
