| Product Name | Cardiac Troponin I antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal [P4-14G5] to cardiac Troponin ITroponin I is part of a heteromeric complex playing an important role in the regulation of skeletal and cardiac muscle contraction. It consists of three subunits, troponin I (TnI), troponin T (TnT) and troponin C (TnC). Each subunit is responsible for part of troponin complex function. E.g. TnI inhibits ATP-ase activity of acto-myosin. TnT and TnI are presented in cardiac muscles in different forms than in skeletal muscles. Only one tissue-specific isoform of TnI is described for cardiac muscle tissue (cTnI). cTnI is expressed only in myocardium. |
| Immunogen | Free human cardiac troponin and/or native troponin complex. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | P4-14G5 |
| Host Species | Mouse |
| Tested Applications | PurifyELISAWB |
| WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Synonyms | 7137 CMH7 TNNI3 Cardiac Troponin I cTnI 115210 P19429 TNNC1 MGC116817 |
|---|---|
| Target | Cardiac Troponin I |
| Tissue Specificity | This antibody is reacting with free cardiac troponin I (cTnI) and cTnI forming complexes with other troponin components (In the presence of 5 mM EDTA). It is not affected by heparin, phosphorylation, oxidation and troponin complex formation.This antibody does not cross-react with skeletal muscle troponin I. |
| Application Notes | WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution, aliquot and store at 4°C. Do not freeze. We recommend to parafilm vials during storage to prevent evaporation of buffer. |
| Storage Buffer | Preservative: 0.1% Sodium Azide; Constituents: PBS. pH 7.4 |
