Application
Sample (30 ug of whole cell lysate) A: A431 B: Jurkat 10% SDS PAGE diluted at 1:1000Application
Immunofluorescence analysis of paraformaldehyde-fixed A431, using CARP antibody at 1:200 dilution.Application
Sample (50 ug of whole cell lysate) A: mouse heart 10% SDS PAGE diluted at 1:1000| Product Name | CARP antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to CARP (ankyrin repeat domain 1 (cardiac muscle))The protein encoded by this gene is localized to the nucleus of endothelial cells and is induced by IL-1 and TNF-alpha stimulation. Studies in rat cardiomyocytes suggest that this gene functions as a transcription factor. Interactions between this protein and the sarcomeric proteins myopalladin and titin suggest that it may also be involved in the myofibrillar stretch-sensor system. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human CARP. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | HumanMouse |
| Predicted Reactivity | Rat, Pig, Rabbit, Sheep, BovineRat (95%), Pig (96%), Rabbit (95%), Sheep (97%), Bovine (95%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | ALRP antibody C-193 antibody CARP antibody CVARP antibody MCARP antibody bA320F15.2 antibody ANKRD1 antibody cytokine-inducible nuclear protein antibody liver ankyrin repeat domain 1 antibody ankyrin repeat domain-containing protein 1 antibody cy |
|---|---|
| Molecular Weight(MW) | 36 kDa (note) (2) |
| Cellular Localization | Nucleus |
Application
Sample (30 ug of whole cell lysate) A: A431 B: Jurkat 10% SDS PAGE diluted at 1:1000
Application
Immunofluorescence analysis of paraformaldehyde-fixed A431, using CARP antibody at 1:200 dilution.
Application
Sample (50 ug of whole cell lysate) A: mouse heart 10% SDS PAGE diluted at 1:1000| Positive Control | 293T , A431 , Jurkat , Raji , mouse heart |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
