Application
Western blot analysis of extracts of various cell lines, using CARS antibody.Application
Immunofluorescence analysis of HeLa cell using CARS antibody. Blue: DAPI for nuclear staining.Application
Immunohistochemistry of paraffin-embedded mouse heart using CARS antibody at dilution of 1:100 (x40 lens).| Product Name | CARS antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to CARSThis gene encodes a class 1 aminoacyl-tRNA synthetase, cysteinyl-tRNA synthetase. Each of the twenty aminoacyl-tRNA synthetases catalyzes the aminoacylation of a specific tRNA or tRNA isoaccepting family with the cognate amino acid. This gene is one of several located near the imprinted gene domain on chromosome 11p15.5, an important tumor-suppressor gene region. Alterations in this region have been associated with Beckwith-Wiedemann syndrome, Wilms tumor, rhabdomyosarcoma, adrenocortical carcinoma, and lung, ovarian and breast cancers. Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq, Aug 2010] |
| Immunogen | Recombinant protein of human CARS |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHCIHC-PWB |
| WB 1:500 - 1:2000, IHC 1:50 - 1:200, IF 1:10 - 1:100: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | CYSRS CARS1 MGC:11246 cysteinyl-tRNA synthetase |
|---|---|
| Target | CARS |
| Molecular Weight(MW) | 85 kDa (note) |
Application
Western blot analysis of extracts of various cell lines, using CARS antibody.
Application
Immunofluorescence analysis of HeLa cell using CARS antibody. Blue: DAPI for nuclear staining.
Application
Immunohistochemistry of paraffin-embedded mouse heart using CARS antibody at dilution of 1:100 (x40 lens).| Application Notes | WB 1:500 - 1:2000, IHC 1:50 - 1:200, IF 1:10 - 1:100: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃. Avoid freeze / thaw cycles. |
| Storage Buffer | PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
