Application
Western blot analysis of CASC3 (arrow) using rabbit polyclonal CASC3 Antibody (Mouse C-term) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the CASC3 gene (Lane 2)Application
Formalin-fixed and paraffin-embedded human breast carcinoma tissue reacted with CASC3 Antibody (C-term) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.| Product Name | CASC3 antibody, C-term |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal to CASC3 (C-term)Component of a splicing-dependent multiprotein exon junction complex (EJC) deposited at splice junction on mRNAs. The EJC is a dynamic structure consisting of a few core proteins and several more peripheral nuclear and cytoplasmic associated factors that join the complex only transiently either during EJC assembly or during subsequent mRNA metabolism. Core components of the EJC, that remains bound to spliced mRNAs throughout all stages of mRNA metabolism, functions to mark the position of the exon-exon junction in the mature mRNA and thereby influences downstream processes of gene expression including mRNA splicing, nuclear mRNA export, subcellular mRNA localization, translation efficiency and nonsense-mediated mRNA decay (NMD). Stimulates the ATPase and RNA-helicase activities of EIF4A3. Plays a role in the stress response by participating in cytoplasmic stress granules assembly and by favouring cell recovery following stress. Binds spliced mRNA in sequence-independent manner, 2-24 nucleotides upstream of mRNA exon-exon junctions. Binds poly(G) and poly(U) RNA homopolymer. Component of the dendritic ribonucleoprotein particles (RNPs) in hippocampal neurons. May play a role in mRNA transport. |
| Immunogen | KLH conjugated synthetic peptide between 210~240 amino acids from the C-terminal region of mouse CASC3. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| ELISA: Use at 1:1,000: |
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| Species Reactivity | Human |
| Concentration | 0.2mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | Metastatic lymph node protein 51 homolog Cancer susceptibility candidate gene 3 protein homolog Cancer susceptibility candidate gene3 protein homolog Metastatic lymph node protein51 homolog Protein MLN 51 homolog Protein MLN51 homolog Protein barents |
|---|---|
| Target | CASC3 |
| Molecular Weight(MW) | 75.639 kDa (note) |
| Cellular Localization | Travels to the cytoplasm as part of the exon junction complex (EJC) bound to mRNA. Shuttles between the cytoplasm and the stress granules. More specifically found in nuclear intrachromatin granules clusters (IGC), also called nuclear speckles, which are s |
Application
Western blot analysis of CASC3 (arrow) using rabbit polyclonal CASC3 Antibody (Mouse C-term) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the CASC3 gene (Lane 2)
Application
Formalin-fixed and paraffin-embedded human breast carcinoma tissue reacted with CASC3 Antibody (C-term) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.| Application Notes | ELISA: Use at 1:1,000: |
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| Form | Liquid |
|---|---|
| Storage Instructions | Store at at 2-8°C for up to 6 months. For long term storage, aliquot and store at -20 °C. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | PBS with 0.09% (W/V) sodium azide |
