Application
Western blot of CK2a (Phospho- Thr360/Ser362) antibody and CK2a antibody in vitro kinase assay. Both purified ERK2 and CK2 were used.| Product Name | Casein Kinase 2 alpha (phospho Thr360/Ser362) antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to CK2 alpha (Phospho Thr360/Ser362)Casein kinases are operationally defined by their preferential utilization of acidic proteins such as caseins as substrates. The alpha and alpha' chains contain the catalytic site. Participates in Wnt signaling. CK2 phosphorylates 'Ser-392' of p53/TP53 following UV irradiation. |
| Immunogen | Peptide sequence around phosphorylation site of threonine360/serine 362 (V-P-T(p)-P-S(p)-P-L) derived from Human CK2 alpha . |
| Modification | p-Thr360/Ser362 |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | WB |
| Suggested starting dilutions are as follows. WB: 1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | CAP102 FLJ36832 FLJ52416 CTNNA1 cadherin-associated protein 102kDa alpha-catenin alphaE-catenin catenin alpha-1 renal carcinoma antigen NY-REN-13 alpha-E-catenin alpha E-catenin catenin (cadherin-associated protein) alpha 1 102kDa |
|---|---|
| Target | CK2 alpha (Phospho Thr360/Ser362) |
| Molecular Weight(MW) | 42 (note) |
Application
Western blot of CK2a (Phospho- Thr360/Ser362) antibody and CK2a antibody in vitro kinase assay. Both purified ERK2 and CK2 were used.| Application Notes | Suggested starting dilutions are as follows. WB: 1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
