Application
Western blot of CK2a(Phospho-Thr360/Ser362) antibody and CK2aantibody in vitro kinase assay. Both purified ERK2 and CK2 were used| Product Name | Casein Kinase 2 alpha antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to CK2 alphaCasein kinases are operationally defined by their preferential utilization of acidic proteins such as caseins as substrates. The alpha and alpha' chains contain the catalytic site. Participates in Wnt signaling. CK2 phosphorylates 'Ser-392' of p53/TP53 following UV irradiation. |
| Immunogen | Peptide sequence around aa. 353~357 (C-S-G-I-S) derived from Human CK2 alpha . |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | WB |
| Suggested starting dilutions are as follows. WB: 1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Target | CK2 alpha |
|---|---|
| Molecular Weight(MW) | 42 (note) |
Application
Western blot of CK2a(Phospho-Thr360/Ser362) antibody and CK2aantibody in vitro kinase assay. Both purified ERK2 and CK2 were used| Application Notes | Suggested starting dilutions are as follows. WB: 1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
