Application
Sample(30 µg whole cell lysate) A: 293T B: A431 C: H1299 D: HeLa S3 E: HepG2 F: MOLT4 G: Raji 10% SDS PAGE diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
Casein Kinase 2 alpha antibody detects CSNK2A1 protein by western blot analysis. A. 50 µg mouse brain lysate/extract 10% SDS-PAGE Casein Kinase 2 alpha antibody dilution: 1:500 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
Immunohistochemical analysis of paraffin-embedded Cal27 xenograft, using Casein Kinase 2 alpha 1 antibody at 1:100 dilution.Application
Casein Kinase 2 alpha antibody detects CSNK2A1 protein at cytoplasm and nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: CSNK2A1 protein stained by Casein Kinase 2 alpha antibody diluted at 1:500. Blue: Hoechst 33342 staining.Application
Rat tissue extract (50 µg) was separated by 10% SDS-PAGE, and the membrane was blotted with Casein Kinase 2 alpha antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
Various whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with Casein Kinase 2 alpha antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.| Product Name | Casein Kinase 2 alpha antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to Casein Kinase 2 alpha (casein kinase 2, alpha 1 polypeptide)Casein kinase II is a serine/threonine protein kinase that phosphorylates acidic proteins such as casein. The kinase exists as a tetramer and is composed of an alpha, an alpha-prime, and two beta subunits. The alpha subunits contain the catalytic activity while the beta subunits undergo autophosphorylation. The protein encoded by this gene represents the alpha subunit. While this gene is found on chromosome 20, a related transcribed pseudogene is found on chromosome 11. Three transcript variants encoding two different proteins have been found for this gene. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human Casein Kinase 2 alpha. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | HumanMouseRat |
| Predicted Reactivity | Zebrafish, Xenopus laevis, Rabbit, Chicken, Rhesus Monkey, BovineZebrafish (80%), Xenopus laevis (92%), Rabbit (100%), Chicken (97%), Rhesus Monkey (100%), Bovine (100%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | CK2A1 antibody CKII antibody CSNK2A1 antibody casein kinase II alpha 1 subunit antibody CK II alpha antibody protein kinase CK2 antibody CK2 catalytic subunit alpha antibody casein kinase II subunit alpha antibody casein kinase 2 alpha 1 polypepti |
|---|---|
| Molecular Weight(MW) | 45 kDa (note) (4) |
Application
Sample(30 µg whole cell lysate) A: 293T B: A431 C: H1299 D: HeLa S3 E: HepG2 F: MOLT4 G: Raji 10% SDS PAGE diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
Casein Kinase 2 alpha antibody detects CSNK2A1 protein by western blot analysis. A. 50 µg mouse brain lysate/extract 10% SDS-PAGE Casein Kinase 2 alpha antibody dilution: 1:500 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
Immunohistochemical analysis of paraffin-embedded Cal27 xenograft, using Casein Kinase 2 alpha 1 antibody at 1:100 dilution.
Application
Casein Kinase 2 alpha antibody detects CSNK2A1 protein at cytoplasm and nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: CSNK2A1 protein stained by Casein Kinase 2 alpha antibody diluted at 1:500. Blue: Hoechst 33342 staining.
Application
Rat tissue extract (50 µg) was separated by 10% SDS-PAGE, and the membrane was blotted with Casein Kinase 2 alpha antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
Various whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with Casein Kinase 2 alpha antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.| Positive Control | 293T , A431 , H1299 , HeLaS3 , HepG2 , Molt-4 , Raji , mouse brain , PC-12 , Rat2 , rat brain |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
