| Product Name | CASK antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal to CASKCalmodulin sensitive kinase (CASK/mLIN-2) is a ~112-kDa member of the membrane associated quanylate kinase (MAGUK) protein family with multiple protein binding domains. These include an N-terminal calmodulin kinase (CaMKII) domain and C-terminal PDZ, SH3-, and quanylate kinase domains. CASK possesses both tyrosine kinase and serine/threonine kinase activity. In addition, CASK can form a heterotrimeric complex with Mint (X11or mLIN-10) and Veli (mLIN-7) proteins which is predicted to participate in plasma membrane receptor localization. CASK is localized to neuronal synapses and also interacts with syndecan and neurexin. The guanylate kinase domain of CASK has been shown to interact with the T-box transcription factor, Tbr-1, as a coactivator to induce transcription of genes that contain T-elements. |
| Immunogen | Synthetic peptide: KSSPQIRNPPSDAVQRA conjugated to KLH, corresponding to amino acids 393-409 of Human CASK. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IPWB |
| IP: Use at a concentration of 5 - 10 μg/ml. WB: Use at a dilution of 1/200. Detects a band of approximately 112 kDa. Optimal dilutions/concentrations should be determined by the end user.: |
|
| Species Reactivity | MouseDogRat |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | 300172 8573 CASK O14936 LIN2 |
|---|---|
| Target | CASK |
| Tissue Specificity | Anti-CASK/Lin2 specifically recognizes human CASK/Lin2 by immunoblotting and immunoprecipitation (112 kDa). Staining of Cask/Lin2 by immunoblotting is inhibited by the immunizing peptide. Additional bands may be detected in some extract preparations. |
| Cellular Localization | Cytoplasmic (Potential). |
| Application Notes | IP: Use at a concentration of 5 - 10 μg/ml. WB: Use at a dilution of 1/200. Detects a band of approximately 112 kDa. Optimal dilutions/concentrations should be determined by the end user.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Preservative: 15mM Sodium Azide; Constituents: 1% BSA, 0.01M PBS. pH 7.4 |
