Application
Various tissue extracts (50 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with CASK antibody diluted at 1:5000.Application
CASK antibody detects CASK protein at cytoplasm in rat kidney by immunohistochemical analysis. Sample: Paraffin-embedded rat kidney. CASK antibody diluted at 1:500.Application
CASK antibody detects CASK protein at cytoplasm in mouse colon by immunohistochemical analysis. Sample: Paraffin-embedded mouse colon. CASK antibody diluted at 1:500.| Product Name | CASK antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to CASK (calcium/calmodulin-dependent serine protein kinase (MAGUK family))Multidomain scaffolding protein with a role in synaptic transmembrane protein anchoring and ion channel trafficking. Contributes to neural development and regulation of gene expression via interaction with the transcription factor TRB1. Binds to cell-surface proteins, including amyloid precursor protein, neurexins, and syndecans. May mediate a link between the extracellular matrix and the actin cytoskeleton via its interaction with syndecan and with the actin/spectrin-binding protein 4.1. |
| Immunogen | Recombinant protein encompassing a sequence within the center region of mouse CASK. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
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| Species Reactivity | MouseRat |
| Predicted Reactivity | Human, Xenopus laevis, Rhesus Monkey, BovineHuman (100%), Xenopus laevis (92%), Rhesus Monkey (93%), Bovine (93%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | DXPri1 antibody DXRib1 antibody LIN2 antibody Pals3 antibody calcium/calmodulin-dependent serine protein kinase (MAGUK family) antibody mLin2 antibody |
|---|---|
| Target | CASK |
| Molecular Weight(MW) | 105 kDa (note) (4) |
| Cellular Localization | Nucleus , Cytoplasm , Cell membrane; Peripheral membrane protein , Nucleus , Cytoplasm , Cell membrane |
Application
Various tissue extracts (50 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with CASK antibody diluted at 1:5000.
Application
CASK antibody detects CASK protein at cytoplasm in rat kidney by immunohistochemical analysis. Sample: Paraffin-embedded rat kidney. CASK antibody diluted at 1:500.
Application
CASK antibody detects CASK protein at cytoplasm in mouse colon by immunohistochemical analysis. Sample: Paraffin-embedded mouse colon. CASK antibody diluted at 1:500.| Positive Control | mouse brain , rat brain |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative. |
