Application
Western blot analysis of Caspase 1 in HeLa cell lysate using at 0.5 ug/ml.Application
Immunohistochemical analysis of Caspase-1 using a polyclonal antibody. Formalin-fixed, paraffin-embedded section of human reactive lymph node stained for Caspase-1 expression using a DAB chromogen and hematoxylin counterstain. B is a higher magnification of A. Staining in macrophages is observed.| Product Name | Caspase 1 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal to Caspase 1Caspases are a family of cysteine proteases that are key mediators of programmed cell death or apoptosis. The precursor form of all caspases is composed of a prodomain, and large and small catalytic subunits. The active forms of caspases are generated by several stimuli including ligand-receptor interactions, growth factor deprivation and inhibitors of cellular functions. All known caspases require cleavage adjacent to aspartates to liberate one large and one small subunit, which associate into a2b2 tetramer to form the active enzyme. Caspase 1 is similar to the cell death gene CED3 of C. elegans and regulates multiple proinflammatory cytokines, including Interleukin 1b and interferon-gamma-inducing factor. Caspase 1 plays a role in down stream of Caspase 8 which is involved in Fas-mediated apoptosis. |
| Immunogen | A synthetic peptide corresponding to amino acids 371-390 RKVRFSFEQPDGRAQMPTTE of human Caspase-1. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| WB: Use at a concentration of 0.5 - 2 μg/ml. Detects a band of approximately 45 kDa. Optimal dilutions/concentrations should be determined by the end user.: |
|
| Species Reactivity | HumanMouse |
| Concentration | 0.5mg/ml |
| Purification | Protein G |
| Gene Synonyms | 147678 IL1BC ICE P29466 P45 Caspase1 Caspase 1 834 CASP1 |
|---|---|
| Target | Caspase 1 |
| Cellular Localization | Cytoplasmic |
Application
Western blot analysis of Caspase 1 in HeLa cell lysate using at 0.5 ug/ml.
Application
Immunohistochemical analysis of Caspase-1 using a polyclonal antibody. Formalin-fixed, paraffin-embedded section of human reactive lymph node stained for Caspase-1 expression using a DAB chromogen and hematoxylin counterstain. B is a higher magnification of A. Staining in macrophages is observed.| Positive Control | HeLa cell lysate |
|---|---|
| Application Notes | WB: Use at a concentration of 0.5 - 2 μg/ml. Detects a band of approximately 45 kDa. Optimal dilutions/concentrations should be determined by the end user.: |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Storage Buffer | Preservative: 0.05% Sodium Azide; Constituents: 0.2% Gelatin, PBS |
