Application
Western blot analysis of extracts of various cell lines, using CASP14 antibody.Application
Immunofluorescence analysis of HeLa cell using CASP14 antibody. Blue: DAPI for nuclear staining.| Product Name | Caspase 14 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to Caspase 14This gene encodes a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme. This caspase has been shown to be processed and activated by caspase 8 and caspase 10 in vitro, and by anti-Fas agonist antibody or TNF-related apoptosis inducing ligand in vivo. The expression and processing of this caspase may be involved in keratinocyte terminal differentiation, which is important for the formation of the skin barrier. [provided by RefSeq, Jul 2008] |
| Immunogen | Recombinant protein of human CASP14 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHCIHC-PWB |
| WB: 1:500 - 1:2000. IHC: 1:50 - 1:200. IF: 1:10 - 1:100: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | caspase 14 CASP14 |
|---|---|
| Target | Caspase 14 |
Application
Western blot analysis of extracts of various cell lines, using CASP14 antibody.
Application
Immunofluorescence analysis of HeLa cell using CASP14 antibody. Blue: DAPI for nuclear staining.| Application Notes | WB: 1:500 - 1:2000. IHC: 1:50 - 1:200. IF: 1:10 - 1:100: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃. Avoid freeze / thaw cycles. |
| Storage Buffer | PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
