Application
Western blot analysis of CASP2 antibody (Center) (Cat. #) in HL60, K562, Ramos and NIH-3T3 cell line lysates(35 µg/lane). CASP2 (arrow) was detected using the purified Pab.Application
CASP2 Antibody (Center) (Cat. #) flow cytometric analysis of HL60 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.Application
Formalin-fixed and paraffin-embedded human lung with CASP2 Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.| Product Name | Caspase 2 antibody, Internal |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal to CASP2 (Internal)This gene encodes a protein which is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme. The proteolytic cleavage of this protein is induced by a variety of apoptotic stimuli. Alternative splicing of this gene results in multiple transcript variants that encode different isoforms. [provided by RefSeq] |
| Immunogen | KLH conjugated synthetic peptide between 205~234 amino acids from the Center region of human Caspase 2. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | FACSIHC-PWB |
| ELISA: Use at 1:1,000: |
|
| Species Reactivity | Human |
| Concentration | 0.2mg/ml |
| Gene Synonyms | Caspase2 Caspase2 subunit p12 NEDD2 Caspase-2 subunit p18 Neural precursor cell expressed developmentally downregulated protein2 Caspase-2 CASP2 Caspase-2 subunit p13 ICH1 protease Caspase2 subunit p18 Neural precursor cell expressed developmenta |
|---|---|
| Target | CASP2 |
| Molecular Weight(MW) | 50.685 kDa (note) |
Application
Western blot analysis of CASP2 antibody (Center) (Cat. #) in HL60, K562, Ramos and NIH-3T3 cell line lysates(35 µg/lane). CASP2 (arrow) was detected using the purified Pab.
Application
CASP2 Antibody (Center) (Cat. #) flow cytometric analysis of HL60 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Application
Formalin-fixed and paraffin-embedded human lung with CASP2 Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.| Application Notes | ELISA: Use at 1:1,000: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at at 2-8°C for up to 6 months. For long term storage, aliquot and store at -20 °C. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | PBS with 0.09% (W/V) sodium azide |
