Application
Immunocytochemistry of caspase-2 in A-20 cells with caspase-2 antibody at 2 μg/mL.Application
Western blot analysis of Caspase-2 in (A) human thymus tissue and (B) human kidney lysate with Caspase-2 antibody at 1 μg/mL.| Product Name | Caspase-2 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Caspase-2 Antibody: Caspases are a family of cysteine proteases that can be divided into the apoptotic and inflammatory caspase subfamilies. Unlike the apoptotic caspases, members of the inflammatory subfamily are generally not involved in cell death but are associated with the immune response to microbial pathogens. Members of this subfamily include caspase-1, -4, -5, and -12 and can activate proinflammatory cytokines such as IL-1β and IL-18. Although phylogenetically similar to this subfamily, Caspase-2 is thought to be involved in stress-induced apoptosis. Caspase-2 has two major isoforms; overexpression on the long form results in apoptosis while that of the short form suppresses cell death.1) Martinon F and Tschopp J. Inflammatory caspases: linking an intracellular innate immune system to autoinflammatory diseases. Cell 2004; 117:561-74. |
| Immunogen | Caspase-2 antibody was raised against a 16 amino acid synthetic peptide from the amino-terminus of human Caspase-2. The immunogen is located within the first 50 amino acids of Caspase-2. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAICCWB |
| Caspase-2 antibody can be used for the detection of caspase-2 by Western blot at 1 μg/mL. Antibody can also be used for immunocytochemistry starting at 2 μg/mL.: |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | CASP2 |
|---|---|
| Alternative Names | Caspase-2 Antibody: ICH1 NEDD2 CASP-2 NEDD-2 PPP1R57 ICH1 Caspase-2 Neural precursor cell expressed developmentally down-regulated protein 2 |
| Molecular Weight(MW) | Predicted: 51 kDa Observed: 50 kDa |
Application
Immunocytochemistry of caspase-2 in A-20 cells with caspase-2 antibody at 2 μg/mL.
Application
Western blot analysis of Caspase-2 in (A) human thymus tissue and (B) human kidney lysate with Caspase-2 antibody at 1 μg/mL.| Application Notes | Caspase-2 antibody can be used for the detection of caspase-2 by Western blot at 1 μg/mL. Antibody can also be used for immunocytochemistry starting at 2 μg/mL.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Caspase-2 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Caspase-2 Antibody is supplied in PBS containing 0.02% sodium azide. |
