Application
WB analysis of HL60 lysate using Caspase 3 antibody [31A893] at a concentration of 2 ug/ml.Application
IHC-P analysis of normal human breast tissue using Caspase 3 antibody [31A893] at a concentration of 5 ug/ml.Application
Western blot analysis of Caspase-3 in Jurkat cells. Cells were treated with 2 uM staurosporine for different time periods. Caspase-3 activation is detected in western blots by the presence of Caspase-3 cleavage fragments. These antibodies detect both pro (full-length) and active (cleaved) Caspase-3, depending on the treatment time points. A basal level of endogenous active Caspase-3 may be see in untreated Jurkat cells.| Product Name | Caspase 3 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal antibody [31A893] to Caspase 3Caspases are a family of cysteine proteases that are key mediators of programmed cell death or apoptosis. The precursor form of all caspases is composed of a prodomain, and large and small catalytic subunits. The active forms of caspases are generated by several stimuli including ligand-receptor interactions, growth factor deprivation and inhibitors of cellular functions. All known caspases require cleavage adjacent to aspartates to liberate one large and one small subunit, which associate into a2b2 tetramer to form the active enzyme. Gene for Caspase 3 also known as Yama, CPP32, and apopain codes for a 32-kDa protein. Caspase 3 cleaves the death substrate poly(ADP-ribose) polymerase (PARP) to a specific 85 kDa form observed during apoptosis and is inhibitable by the CrmA protein. Other Caspase 3 substrates include DNA-PK, actin, GAS2, and procaspase-6, etc. Caspase 3 is activated by cleavage events at Asp-28/Ser-29 (between N-terminal pro-domain) and Asp-175/Ser-176 (between large and small subunits) to generate a large subunit of 17-kDa and a small subunit of 12-kDa. |
| Immunogen | Recombinant fusion protein (6xHis tagged full-length human Caspase 3). |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | 31A893 |
| Host Species | Mouse |
| Tested Applications | IHC-PWB |
| WB: Use at a concentration of 2 μg/ml. Predicted molecular weight: 31 kDa. This antibody only detects a 32 kD caspase 3 corresponding to pro caspase 3. Optimal dilutions/concentrations should be determined by the end user.: |
|
| Species Reactivity | Human |
| Concentration | 0.5mg/ml |
| Purification | Protein G |
| Gene Synonyms | pro Caspase3 pro Caspase 3 CPP32 CPP32B SCA-1 SCA 1 SCA1 APOPAIN caspase 3 preproprotein CPP32 cysteine protease CPP32 PARP cleavage protease SREBP cleavage activity 1 Yama |
|---|---|
| Target | Caspase 3 |
| Cellular Localization | Cytoplasmic |
Application
WB analysis of HL60 lysate using Caspase 3 antibody [31A893] at a concentration of 2 ug/ml.
Application
IHC-P analysis of normal human breast tissue using Caspase 3 antibody [31A893] at a concentration of 5 ug/ml.
Application
Western blot analysis of Caspase-3 in Jurkat cells. Cells were treated with 2 uM staurosporine for different time periods. Caspase-3 activation is detected in western blots by the presence of Caspase-3 cleavage fragments. These antibodies detect both pro (full-length) and active (cleaved) Caspase-3, depending on the treatment time points. A basal level of endogenous active Caspase-3 may be see in untreated Jurkat cells.| Positive Control | HL60 whole cell lysates, NIH 3T3 |
|---|---|
| Application Notes | WB: Use at a concentration of 2 μg/ml. Predicted molecular weight: 31 kDa. This antibody only detects a 32 kD caspase 3 corresponding to pro caspase 3. Optimal dilutions/concentrations should be determined by the end user.: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Store at 4ºC short term. For extended storage aliquot and store at -20ºC or below. Avoid freeze-thaw cycles. |
| Storage Buffer | Preservative: 0.02% Sodium Azide; Constituents: PBS |
