Mouse monoclonal [3CSP03] to Caspase 3Caspase 3 is associated with induction of apoptosis. Caspase 3 is ubiquitously express and is synthesized as an inactive proenzyme.
Immunogen
Recombinant full length protein (Human).
Key Feature
Clonality
Monoclonal
Isotype
IgG2a
Clone Number
3CSP03
Host Species
Mouse
Tested Applications
IHCIHC-FIHC-PIPWB
IHC: This antibody may be diluted to a titer of 1:25-1:75 in an ABC method. We suggest an incubation period of 30 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation conditions and antibody dilutions should be determined by the user. Formalin fixed paraffin embedded tissue sections require high temperature antigen unmasking with all10 mM citrate buffer, pH 6.0 prior to immunostaining.:
This antibody is specific to a 32 kDa protein, which is identified as Caspase 3.
Cellular Localization
Cytoplasmic
Application
Application
IHC-P analysis of human tonsil stained using Caspase 3 antibody.
Application
IHC image using - Detection of Caspase 3 by IHC in human tonsil
Positive Control
Tonsil
Application Notes
IHC: This antibody may be diluted to a titer of 1:25-1:75 in an ABC method. We suggest an incubation period of 30 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation conditions and antibody dilutions should be determined by the user. Formalin fixed paraffin embedded tissue sections require high temperature antigen unmasking with all10 mM citrate buffer, pH 6.0 prior to immunostaining.: