| Product Name | Caspase 3 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal [3F49] to Caspase 3The caspase family of cysteine proteases play a key role in apoptosis. Caspase 3 (also known as CPP32, YAMA and apopain) is the most extensively studied apoptotic protein among caspase family members. Caspase 3 is synthesized as inactive pro-enzyme that is processed in cells undergoing apoptosis by self-proteolysis and/or cleavage by other upstream proteases (e.g. Caspases 8, 9 and 10). The processed form of Caspase 3 consists of large (17kD) and small (12kD) subunits which associate to form an active enzyme. Caspase 3 is cleaved at Asp28 - Ser29 and Asp175 - Ser176. The active Caspase 3 proteolytically cleaves and activates other caspases (e.g. Caspases 6, 7 and 9), as well as relevant targets in the cells (e.g. PARP and DFF). Alternative splicing of this gene results in two transcript variants which encode the same protein. In immunohistochemical studies Caspase 3 expression has been shown to be widespread but not present in all cell types (e.g. commonly reported in epithelial cells of skin, renal proximal tubules and collecting ducts). Differences in the level of Caspase 3 have been reported in cells of short-lived nature (eg germinal centre B cells) and those that are long lived (eg mantle zone B cells).Caspase 3 is the predominant caspase involved in the cleavage of amyloid-beta 4A precursor protein, which is associated with neuronal death in Alzheimer's disease. |
| Immunogen | Recombinant full length protein (Human). |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2a |
| Clone Number | 3F49 |
| Host Species | Mouse |
| Tested Applications | IHC-PIPWB |
| IHC-P: Use at a concentration of 1 - 2 μg/ml. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min. IP: Use 2 μg/mg of protein lysate. Use protein A. WB: Use at a concentration of 1 μg/ml. Predicted molecular weight: 32 kDa. Optimal dilutions/concentrations should be determined by the end user.: |
|
| Species Reactivity | Human |
| Concentration | 0.2mg/ml |
| Purification | Protein A |
| Gene Synonyms | 836 P42574 CASP3 Caspase3 SCA1 Caspase 3 CPP32B CPP32 SCA-1 600636 SCA 1 |
|---|---|
| Target | Caspase 3 |
| Cellular Localization | Cytoplasmic |
| Positive Control | Jurkat cells, tonsil and appendix. |
|---|---|
| Application Notes | IHC-P: Use at a concentration of 1 - 2 μg/ml. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min. IP: Use 2 μg/mg of protein lysate. Use protein A. WB: Use at a concentration of 1 μg/ml. Predicted molecular weight: 32 kDa. Optimal dilutions/concentrations should be determined by the end user.: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Store at 4ºC short term. For extended storage aliquot and store at -20ºC or below. Avoid freeze-thaw cycles. |
| Storage Buffer | Preservative: 0.09% Sodium Azide. Constituents: 0.2% BSA, 10mM PBS. pH 7.4 |
