Application
Immunohistochemistry-Paraffin: Caspase 3 Antibody (CPP32 4-1-18) [ - Rat epithelial cells of the tongue base. Antigen retrieval method: Citrate bufferApplication
Western Blot: Caspase 3 Antibody (CPP32 4-1-18) - Western blot detection of Caspase (19 and 35 kDa) from HEK293 cell extract using . Lanes 1 and 2 contain inactive and active Caspase, respectively| Product Name | Caspase 3 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal ( CPP32 4 - 1 - 18 ) to human Caspase 3Caspases are cysteine proteases, expressed as inactive precursors, that mediate apoptosis by proteolysis of specific substrates. Caspases have the ability to cleave after aspartic acid residues. There are two classes of caspases involved in apoptosis; initiators (activation by receptor cluster) and effectors (activation by mitochondrial permeability transition). Proapoptotic signals autocatalytically activate initiator caspases, such as Caspase-8 and Caspase-9. Activated initiator caspases then process effector caspases, such as Caspase-3 and Caspase-7, which in turn cause cell collapse. Effector Caspase 3 cleaves numerous substrates to effect the morphological changes associated with apoptosis. |
| Immunogen | Full-length recombinant human Caspase 3 protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2a |
| Clone Number | CPP32 4-1-18 |
| Host Species | Mouse |
| Tested Applications | IHC-PWB |
| For WB: Use at a dilution of 1:500 - 1:1,000. May be used for Western analysis where a band is seen at ~32 kDa for the inactive form of Caspase 3 and ~17-22 kDa for the active form of Caspase 3. Optimal dilutions/concentrations should be determined by the researcher.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | 836 P42574 CASP3 Caspase3 SCA1 Caspase 3 CPP32B CPP32 SCA-1 600636 SCA 1 |
|---|---|
| Target | Caspase 3 |
| Tissue Specificity | Specific for Caspase 3. |
| Light Chain Type | kappa |
Application
Immunohistochemistry-Paraffin: Caspase 3 Antibody (CPP32 4-1-18) [ - Rat epithelial cells of the tongue base. Antigen retrieval method: Citrate buffer
Application
Western Blot: Caspase 3 Antibody (CPP32 4-1-18) - Western blot detection of Caspase (19 and 35 kDa) from HEK293 cell extract using . Lanes 1 and 2 contain inactive and active Caspase, respectively| Positive Control | Human kidney 293 cell lysates |
|---|---|
| Application Notes | For WB: Use at a dilution of 1:500 - 1:1,000. May be used for Western analysis where a band is seen at ~32 kDa for the inactive form of Caspase 3 and ~17-22 kDa for the active form of Caspase 3. Optimal dilutions/concentrations should be determined by the researcher.: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Store at 4ºC short term. For extended storage aliquot and store at -20ºC or below. Avoid freeze-thaw cycles. |
| Storage Buffer | Ascitic fluid containing 0.1% sodium azide |
