Application
Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Caspase 4 antibody diluted at 1:1000.Application
Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Caspase 4 antibody diluted at 1:1000.Application
Caspase 4 antibody detects Caspase 4 protein at cytoplasm in human cervical carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human cervical carcinoma. Caspase 4 antibody diluted at 1:500.Application
Sample (30 ug of whole cell lysate) A: H1299 10% SDS PAGE diluted at 1:1000Application
Caspase 4 antibody detects CASP4 protein at cytosol on SAS xenograft by immunohistochemical analysis. Sample: Paraffin-embedded SAS xenograft. Caspase 4 antibody dilution: 1:500.| Product Name | Caspase 4 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to Caspase 4 (caspase 4, apoptosis-related cysteine peptidase)This gene encodes a protein that is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes composed of a prodomain and a large and small protease subunit. Activation of caspases requires proteolytic processing at conserved internal aspartic residues to generate a heterodimeric enzyme consisting of the large and small subunits. This caspase is able to cleave and activate its own precursor protein, as well as caspase 1 precursor. When overexpressed, this gene induces cell apoptosis. Alternative splicing results in transcript variants encoding distinct isoforms. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human Caspase 4. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | Human |
| Predicted Reactivity | Rhesus Monkey, BovineRhesus Monkey (96%), Bovine (84%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | ICE(rel)II antibody ICEREL-II antibody ICH-2 antibody Mih1/TX antibody TX antibody CASP4 antibody apoptotic cysteine protease Mih1/TX antibody CASP-4 antibody caspase 4 apoptosis-related cysteine protease antibody protease ICH-2 antibody proteas |
|---|---|
| Molecular Weight(MW) | 43 kDa (note) (3) |
Application
Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Caspase 4 antibody diluted at 1:1000.
Application
Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Caspase 4 antibody diluted at 1:1000.
Application
Caspase 4 antibody detects Caspase 4 protein at cytoplasm in human cervical carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human cervical carcinoma. Caspase 4 antibody diluted at 1:500.
Application
Sample (30 ug of whole cell lysate) A: H1299 10% SDS PAGE diluted at 1:1000
Application
Caspase 4 antibody detects CASP4 protein at cytosol on SAS xenograft by immunohistochemical analysis. Sample: Paraffin-embedded SAS xenograft. Caspase 4 antibody dilution: 1:500.| Positive Control | H1299 , K562 , THP-1 , HL-60 , myc-tagged human caspas4-transfected 293T cells |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
