Application
Western blot analysis of extracts from 293 cells, treated with etoposide 25uM 1h, using Caspase 8 (Cleaved-Asp384) Antibody. The lane on the right is treated with the synthesized peptide.| Product Name | Caspase 8 (cleaved Asp384) antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal antibody to CASP8 (cleaved Asp384)Most upstream protease of the activation cascade of caspases responsible for the TNFRSF6/FAS mediated and TNFRSF1A induced cell death. Binding to the adapter molecule FADD recruits it to either receptor. The resulting aggregate called death-inducing signaling complex (DISC) performs CASP8 proteolytic activation. The active dimeric enzyme is then liberated from the DISC and free to activate downstream apoptotic proteases. Proteolytic fragments of the N-terminal propeptide (termed CAP3, CAP5 and CAP6) are likely retained in the DISC. Cleaves and activates CASP3, CASP4, CASP6, CASP7, CASP9 and CASP10. May participate in the GZMB apoptotic pathways. Cleaves ADPRT. Hydrolyzes the small-molecule substrate, Ac-Asp-Glu-Val-Asp-|-AMC. Likely target for the cowpox virus CRMA death inhibitory protein. Isoform 5, isoform 6, isoform 7 and isoform 8 lack the catalytic site and may interfere with the pro-apoptotic activity of the complex. |
| Immunogen | The antiserum was produced against synthesized peptide derived from human Caspase 8. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAWB |
| ELISA: 1:40000Optimal dilutions should be determined experimentally by the end user.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | Casp8 FLICE FLJ17672 CAP4 MGC78473 MACH MCH5 ALPS2B CASP8 (cleaved D384) CASP8 (cleaved Asp384) CASP8 cleaved D384 CASP8 cleaved Asp384 Cleaved CASP8 (D384) Cleaved CASP8 (Asp384) |
|---|---|
| Target | CASP8 |
| Molecular Weight(MW) | 47 and 55 kDa (note) |
| Tissue Specificity | Caspase 8 (Cleaved-Asp384) Antibody detects endogenous levels of fragment of activated Caspase 8 resulting from cleavage adjacent to Asp384. |
Application
Western blot analysis of extracts from 293 cells, treated with etoposide 25uM 1h, using Caspase 8 (Cleaved-Asp384) Antibody. The lane on the right is treated with the synthesized peptide.| Application Notes | ELISA: 1:40000Optimal dilutions should be determined experimentally by the end user.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20 degrees Celcius. Product is stable for at least 1 year. |
| Storage Buffer | Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
