Application
ELISA analysis of control antigen and antigen using CASP8 antibody [1H11].Application
WB analysis of (1) HeLa, (2) Jurkat, (3) THP-1, (4) NIH3T3, (5) Cos7 and (6) PC-12 lysates using CASP8 antibody [1H11].Application
IHC-P analysis of liver cancer tissues using CASP8 antibody [1H11].Application
IHC-P analysis of colon cancer tissues using CASP8 antibody [1H11].Application
FACS analysis of NIH3T3 cells using CASP8 antibody [1H11] (blue) or a negative control (red).| Product Name | Caspase 8 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal antibody [1H11] to Caspase 8This gene encodes a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes composed of a prodomain, a large protease subunit, and a small protease subunit. Activation of caspases requires proteolytic processing at conserved internal aspartic residues to generate a heterodimeric enzyme consisting of the large and small subunits. This protein is involved in the programmed cell death induced by Fas and various apoptotic stimuli. The N-terminal FADD-like death effector domain of this protein suggests that it may interact with Fas-interacting protein FADD. This protein was detected in the insoluble fraction of the affected brain region from Huntington disease patients but not in those from normal controls, which implicated the role in neurodegenerative diseases. Many alternatively spliced transcript variants encoding different isoforms have been des |
| Immunogen | The immunogen corresponding to a region within amino acids 302 and 464 of human caspase 8. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | 1H11 |
| Host Species | Mouse |
| Tested Applications | ELISAFACSIHCIHC-PWB |
| ELISA: 1/10000.: |
|
| Species Reactivity | HumanMouseMonkeyRat |
| Concentration | 1mg/ml |
| Gene Synonyms | CASP-8 ALPS2B MCH5 CAP4 FLICE MACH CASP8 |
|---|---|
| Target | Caspase 8 |
| Molecular Weight(MW) | 26 kDa (note) |
Application
ELISA analysis of control antigen and antigen using CASP8 antibody [1H11].
Application
WB analysis of (1) HeLa, (2) Jurkat, (3) THP-1, (4) NIH3T3, (5) Cos7 and (6) PC-12 lysates using CASP8 antibody [1H11].
Application
IHC-P analysis of liver cancer tissues using CASP8 antibody [1H11].
Application
IHC-P analysis of colon cancer tissues using CASP8 antibody [1H11].
Application
FACS analysis of NIH3T3 cells using CASP8 antibody [1H11] (blue) or a negative control (red).| Application Notes | ELISA: 1/10000.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | 4°C; -20°C for long term storage |
| Storage Buffer | Ascitic fluid containing 0.03% sodium azide. |
